摘要
目的探讨急性髓系白血病患者化疗早期细胞凋亡率与疾病预后的关系。方法 (1)所有对象均来源于2011年7月至2012年10月在潍坊医学院附属医院血液科接受住院化疗的初发AML患者20例;(2)所有患者均采用DA或IDA或HA方案诱导缓解;(3)应用流式细胞术(FCM)及AnnexinV-FITC/PI染色法检测患者化疗前1 d及化疗第2 d白血病细胞凋亡率。结果 (1)经诱导治疗7 d后,完全缓解(complete remission,CR)14例,未缓解(not remission,NR)6例;(2)化疗第2 d白血病细胞凋亡率(9.31±2.42)%明显高于化疗前1 d(4.41±2.13)%,(P<0.05);(3)化疗后CR组患者细胞凋亡率(10.12±2.23)%明显高于NR组(7.43±2.74)%(P<0.05)。结论 化疗早期细胞凋亡率可作为预测急性髓系白血病疗效的指标之一。
Objective To explore the relationship between the apoptosis rate in the early period of chemotherapy for acute myelogenous leukemia and the post - treatment. Method Firstly, the subjects were 20 primary AML inpatients re- ceiving chemotherapy in Department of Hematology of Weifang Medical University from July 2011 to October 2012, then. All the patients underwent remission induction by using DA, IDA or HA scheme; lastly, the flow cytometry （FCM） and the AnnexinV - FITC/H staining method were applied to detect the leukemia cell apoptosis rate the day before chemothera- py and the day after. Result （ 1 ） After seven days＇ inductive treatment, 14 cases were in complete remission, and 6 ca- ses were not. （2） On the second day of the chemotherapy, the leukemia cell apoptosis rate （9.31 ±2.42 ）% was obvious- ly higher than that of the day before the chemotherapy （4.41 ± 2. 13 ）%, （P 〈 0. 05 ）. （3） After the chemotherapy, the apoptosis rate （10. 12 ±2. 23）% of those in the CR （complete remission） group is obviously higher than the NR （not re- mission） group （7. 43 ± 2. 74） % （ P 〈 0. 05 ）. Conclusion The apoptosis rate at the early stage of chemotherapy can be used as one of indexes for the curative prediction of acute myelogenous leukemia.
出处
《辽宁医学院学报》
CAS
2013年第2期44-45,共2页
Journal of Liaoning Medical University (LNMU) Bimonthly
关键词
急性髓系白血病
细胞凋亡
预后
acute myelogenous leukemia
cell apoptosis
post- treatment
