摘要
目的研究金银花和野菊花乙醇提取物抑菌作用的差异。方法采用抑菌环试验对它们的抑菌作用进行观察比较。结果生药含量为492mg/ml金银花乙醇提取物对金黄色葡萄球菌、大肠杆菌的抑菌环直径均大于8mm,而对枯草杆菌的抑菌环直径小于8mm;生药含量为1035mg/ml野菊花乙醇提取物对金黄色葡萄球菌、枯草杆菌的抑菌环直径均大于8mm,而对大肠杆菌的抑菌环直径小于8mm;金银花乙醇提取物对金黄色葡萄球菌和枯草杆菌抑菌作用较强,两种菌的最低抑菌浓度皆为1∶8,对大肠杆菌的最低抑菌浓度为1∶4;野菊花乙醇提取物对大肠杆菌抑菌作用最强,最低抑菌浓度为1∶64;对金黄色葡萄球菌的最低抑菌浓度为对枯草杆菌的最低抑菌浓度为1∶16;对枯草杆菌的抑菌作用最弱,最低抑菌浓度为1∶4。结论金银花乙醇提取物对金黄色葡萄球菌、枯草杆菌有抑菌作用,而对大肠杆菌无抑菌作用;野菊花乙醇提取物对金黄色葡萄球菌、大肠杆菌有抑菌作用,而对枯草杆菌无抑菌作用。
Objective The bacteriostatic efficacy of alcohol extract of honeysuckle and wild chrysanthemum were investigated in this work. Methods Their reaction was observed and compared by experiments with Paper Dish methods. Results The diameters of bacterial inhibition zones of two bacteria such as Staphylococcus aureus and Subtilis Bacillus to the ethanol extract containing honeysuckle crude drug 492mg/ml were larger than 8mm, but the diameters of bacterial inhibition zones of Escherichia coliand Bacillus to the ethanol extract containing hon- eysuckle crude drug 492mg/ml were smaller than 8ram. The diameters of bacterial inhibition zones of two bacteria such as Staphylococcus aureus and Escherichia coliand Bacillus to the ethanol extract containing wild chrysanthe- mum drug 1035mg/ml were larger than 8mm, but the diameters of bacterial inhibition zones of Subtilis Bacillus to the ethanol extract containing wild chrysanthemum drug 1035mg/ml were smaller than 8mm. The minimal inhibi- tory concentration was 1 .＂ 8, such as honeysuckle alcohol extract to Staphylococcus aureus and Subtilis Bacillus. The minimal inhibitory concentration was 1 ~ 4 such as honeysuckle alcohol extract to the E. coli. The minimal in- hibitory concentration was 1 .＂ 64 such as wild chrysanthemum alcohol extract to the E. coli. The minimal inhibito- ry concentration was 1 z 16 such as wild chrysanthemum alcohol extract to the Staphylococcus aureus. The mini- mal inhibitory concentration was 1 ~ 4 such as wild chrysanthemum alcohol extract to the Subtilis Bacillus. Conclu- sion The honeysuckle extract has obvious bacteriostatic effect to Staphylococcus aureus and Subtilis Bacillus, but the honeysuckle extract has no inhibitory effect to Escherichia coli and Bacillus. The wild chrysanthemum extract has obvious bacteriostatic effect to Staphylococcus aureus, Escherichia coli and Bacillus, but the honeysuckle ex- tract has no inhibitory effect to Subtilis Bacillus.
出处
《济宁医学院学报》
2013年第2期97-99,105,共4页
Journal of Jining Medical University
关键词
金银花乙醇提取物
野菊花乙醇提取物
抑菌作用
Honeysuckle extract ~ Wild chrysanthemum extract ~ Bacteriostatic efficacy
