摘要
目的 检测 1194例初诊怀疑患强直性脊柱炎 (AS)和其它关节性疾病患者的HLA B2 7、B7、B13和B40位点抗原的分布 ,并分析这些位点的相互杂合和其抗原的交叉反应性。方法 微量细胞毒反应法 ,在同一块反应盘上同时检测。结果 1.在 1194例患者中 ,HLA B2 7抗原阳性率达38 6 % ;B7的抗原阳性率为 7 4% ;B13为 14 6 % ;B40为 13 4%。2 .在HLA B2 7阳性人群中 ,B7抗原的阳性率为 14 3% ,明显升高 ,而在HLA B2 7阴性人群中 ,B7的阳性率仅为 3 2 % ,明显降低 ;3.在44 7例HLA B2 7阳性人群中 ,未检测到HLA B2 7、B7、B13三种抗原皆为阳性者 ,说明B7和B13之间没有交叉 ;4.据此 ,在HLA B2 7阴性人群中 ,查到的HLA B7/B13阳性率 0 5 4%应代表这两个位点的杂合 ;5 .无论在HLA B2 7阳性还是阴性人群中 ,HLA B13/B40的抗原阳性率远高于HLA B7/B40的阳性率 ,说明B13和B40位点的杂合或交叉 ,远大于B7与B40之间的杂合或交叉。结论 检测HLA B2
Objective To estimate the frequencies of HLA B27, B7, B13 and B40 alleles in Chinese patients with ankylosing spondylitis (AS) or related disorders and to analyze heterozygotic frequencies among these loci and the cross reactivities of their antigens. Methods Microcytotoxicity assay. Results 1. The positive rates for HLA B27, B7, B13 and B40 were 38.6%, 7.4%, 14.6% and 13.4%, respectively; 2. In the HLA B27 positive population the frequency of B7 was 14.3%, significantly higher than that (3.2%) in the HLA B27 negative group; 3. None was positive for both B7 and B13 in the 447 HLA B27 positive patients; 4. Therefore, the HLA B7/B13 positive rate of 0.54% in the HLA B27 negative individuals represented the heterozygotic frequency between HLA B7 and B13 locus; 5. In both HLA B27 positive and negative groups, positive rate for HLA B13/B40 was higher than that of HLA B7/B40, implying that the heterozygotic frequency of B13 and B40 loci as well as the cross reactivity of their antigens or both was higher. Conclusion Detection of HLA B27 and its related B antigens was useful to investigate the heterozygosis of these B loci and the cross reactivity of their antigens.
出处
《中华微生物学和免疫学杂志》
CSCD
北大核心
2000年第5期389-392,共4页
Chinese Journal of Microbiology and Immunology
