摘要
甲烷氧化菌素(methanobactin,mb)是具有过氧化氢还原酶活性的荧光肽.从甲基弯菌Methylosinus trichospo-rium IMV3011限铜培养介质中分离mb,采用紫外可见全波长扫描法观察mb催化对苯二酚还原氯金酸合成纳米金的作用和影响,当mb/氯金酸/对苯二酚反应液中mb的浓度分别是2.5×10-5mol/L、5.0×10-5mol/L和1.0×10-4mol/L时,形成的纳米金溶液的特征峰分别是561.5 nm(OD561=0.158)、548.0 nm(OD5 48=0.426)、536.5 nm(OD5 36=0.541),特征峰波长减小,对应的吸光值增大,表明mb能够催化对苯二酚还原氯金酸合成纳米金,并且可以通过调控mb的浓度控制纳米金的合成量及粒径大小.
Methanobactin（mb） is a fluorescent peptide that appears to function of hydrogen peroxide reductase. The mb was found to be accumulated in the spent medium of Methylosinus trichosporium IMV 3011 under copperlimited condition. The role and impact of mb catalysis on the synthesis of gold nanoparticles by hydroquinone reduction of a chloroauric acid （ HAuCl4 ） was observed by the UV-visible absorption spectra. Characteristic peaks of gold nanoparticles was 561.5 nm （ 0D561 = 0. 158 ） , 548.0 nm （ OD548 = 0. 426） , 536.5 nm （ OD536 = 0. 541 ） when the mb concentration in mixture was 2.5×10-5mol/L, 5.0×10-5 mol/L, 1.0×10-5 mol/L, respectively. Wavelength of characteristic peak decreased with increasing absorption value. The results suggested that the synthesis of gold nanoparticles by hydroquinone reduction of a HAuCl4 can be catalyzed by rob, and the synthetic quantity and the size of gold nanoparticles can be controlled by regulating the concentration of mb as well.
出处
《分子催化》
EI
CAS
CSCD
北大核心
2013年第2期192-197,共6页
Journal of Molecular Catalysis(China)
基金
国家自然科学基金(20873034
21073050)
黑龙江省教育厅重点项目(11551z007)和面上项目(12521138)
关键词
甲烷氧化菌素
催化
对苯酚
纳米金
methanobactin
catalysis
hydroquinone
gold nanopartieles
