摘要
目的 :研究胃癌组织、癌旁及远端正常胃组织中的端粒酶活性 ,探讨其作为胃癌肿瘤标记物的可能性。方法 :采用TRAP-ELISA法检测了 5 1例胃癌组织、癌旁及远端正常胃组织的端粒酶活性表达。结果 :5 1例胃癌组织中端粒酶阳性表达 48例( 94.1%) ,癌旁组织为 17例 ( 3 3 .3 %) ,远端正常胃组织未检测出端粒酶活性。结论 :端粒酶是特异性较强的恶性肿瘤基因标志 ,有可能成为胃癌早期诊断和治疗的理想标记物。
Objective:To investigate the telomerase activity in human gastric carcinoma,tumor aduacent tissue and normal gastric tissue and to investigate the possibility of telomaerase as tumor biological marker.Methods:The expression of telomerase activity in cancer,paracancer and normal tissue in 51 patients with gastric carcinoma were detected by TRAP-ELISA method.Results:The positive rate of telomerase in gastric carcinoma and paracancer is 94.1% (48/51) and 33.3% (17/51),no telomerase activity in 51 normal gastric tissues.Conclusion:The data suggest the telomerase is a tumor specific gene marker,and may play an important role in early diagnosis and treatment of gastric carcinomas. [
出处
《南通医学院学报》
2000年第1期47-48,共2页
ACTA Academiae Medicinae Nantong
关键词
胃癌
端粒
端粒重复序列扩增法
gastric carcinoma
telomere
telomerase
telomeric repeat
amplificationprotocol
