摘要
目的 :建立一种简便、实用的检测大量水样中的微量病毒的方法。方法 :将 L 2 0 B细胞加入大量的含微量脊髓灰质炎病毒的水中 ,充分作用后收集细胞 ,作病毒的分离培养。同时以 Na Cl— Al Cl3沉淀法作对照。结果 :10 0 0 ml水样中含病毒 10 0、10 TCD5 0时能全部检出 ;当含 1— 2 TCD5 0 时 14份样本可检出 13份。 Na Cl— Al Cl3沉淀法 :在 10 0 TCD5 0 时 4份样本中仅检出 1份 ,其它各浓度的 4份样都未能检出。结论 :细胞吸附浓集法分离水中病毒敏感、实用。
Objective: To set up a simple, practical, new method for detecting minimum viruses in large volume water. Methods: Adding L20B cells into the large volume water containing minimum viruses. Collecting the cells after they fully reacted with viruses, then culturing viruses, with the method of NaCl-AlCl 3 sedimentation as control. Results: If the amount of viruses was 100,10TCID 50 per 1000ml water, the viruses could be detected. When the amount was 1～2TCID 50 , the viruses in 13 of 14 samples could be detected .While the viruses ,of 100TCID 50 per 1000ml water in 1 of 4 samples ,could be detected with the method of NaCl-AlCl 3 sedimentation, but the viruses of other concentrations in all samples couldn't be detected. Conclusions: Absorbing viruses in water with the method of the cell absorption was sensitive and adaptive.[
出处
《南通医学院学报》
2000年第1期28-29,共2页
ACTA Academiae Medicinae Nantong
