摘要
酿酒酵母的固定化细胞技术能够实现可连续与重复再利用性强、并且能够实现酿酒酵母细胞的高密度培养。本研究利用海藻酸锰代替海藻酸钙作为固定化酿酒酵母的载体,采用响应面方法优化固定化工艺,为了降低海藻酸锰凝胶球的传质阻力,在凝胶球中添加适量的经过酶解的秸秆纤维素。结果表明,最佳工艺条件为海藻酸钠(SA)初始浓度为2.18%,秸秆纤维素(CS)添加量为0.21%,CaCl2初始浓度为5.21%,在此BBD响应面模型优化条件下,制备固定化酿酒酵母细胞的包埋率理论值可达到88.02%。包埋率从48%提高为88.02%。表明了BBD响应面模型对于制备固定化细胞的工艺流程具有很高的的应用价值。
Saccharomyces cerevisiae immobilized cell technology is able to achieve a continuous and repeated recyclability, and can achieve a high density culture of Saccharomyces cerevisiae cells. In this study, alginate manganese instead of calcium alginate as carrier immobilized Saccharomyces cerevisiae. Using response surface methodology to optimize the immobilization process, in order to reduce the mass transfer resistance of alginic acid the manganese gel ball, add the right amount of enzymed lignocellulose. The results showed that the optimum conditions were as follows： 2.18% the initial concentration of sodium alginate （SA）, the amount of straw cellulose （CS） 0.21%, and 5.21% initial concentration of CaCIz. Using BBD response surface model, the theoretical value of the embedding rate reached 88.02%. The embedding rate increased from 48% to 88.02%. Results suggested that the BBD response surface model has a very high value process for the preparation of immobilized cells.
出处
《中国酿造》
CAS
2013年第4期42-47,共6页
China Brewing
基金
河南工业大学校高层次人才基金(2012BS049)
关键词
秸秆纤维素
固定化
酿酒酵母
响应面法
lignocellulose
immobilized
Saccharomyces cerevisiae
response surface methodology
