摘要
从广西不同地方的发病仔猪脑内分离到两株病毒 ,分别命名为 B株和 W株。用这两株病毒分别接种家兔 ,产生的症状和病变与伪狂犬病病毒 ( Pseudorabies virus)强毒 Min A株相似。将两株病毒在乳兔肾细胞和 CER细胞上连续传代 ,均稳定地出现与 Min A株相同的细胞病变 ( CPE)。在 CER细胞上进行微量血清中和试验 ,Min A株的抗血清能完全中和 B株和 W株对细胞的致病变作用 ,表明两个毒株与 Pr V强毒具有相似的抗原性。电镜下观察 B株和 W株病毒粒子直径为 1 0 0～ 1 90 nm,有囊膜 ,近似圆形 ,与 Min A株的形态和结构相似。根据 Pr V的 gp5 0 和 g I基因序列合成的两对引物 PG1 /PG2 、PI1 /PI2 分别对 Min A株、B株、W株及疫苗株进行 PCR,结果 PG1 /PG2 对各毒株均能特异性扩增出 2 1 7bp的片段 ,PI1 /PI2 对 Min A株、B株和 W株能特异性扩增出 52 6bp的片段 ,而对缺失了 g I基因的疫苗株不能扩增出任何片段。研究结果表明 ,所分离到的两个野毒株 B株和 W株是 Pr
Two field strains of Pseudorabies Virus (PrV) were isolated from brains of dead piglets of different farms in Guangxi,Which were named B and W strains respectively.Rabbits inoculated with B and W strains showed typical syndrome of pseudorabies disease .Both B and W strains could cause the same CPE steadily as MinA strain,when they were passaged serially on PK cells or CER cells.Shape and structure were similar with PrV MinA strain when observed under elctron mi croscope.The results of microserum neutralization test showed that B and W strains had the same antigenicity as PrV MinA strain .In order to effectively distinguish the vaccine and field isolates of PrV,two pairs of primers based on sequences of PrV gp 50 and gI gens have been synthetized.The PG 1/PG 2 primers(from gp 50 ) amplified a fragment with desired size of 217 bp from standard PrV MinA strain,two isolates and vaccine of PrV.Amplification using the primers PI 1/PI 2(from gI) was also subjected to the four strains. However only MinA strain and two field isolates were positive,amplifying a fragment with desired size of 526 bp. PI 1/PI 2 could not amplify any fragment from vac cine strain that a gI gene was deleted.Results of this study proved that the two isolates belonged to virulent virus of PrV.
出处
《广西农业生物科学》
CAS
CSCD
2000年第3期188-191,共4页
Journal of Guangxi Agricultural and Biological Science
