摘要
背景:墨西哥钝口螈脊髓切断可以再生,再生过程伴随胶质细胞数目及分布的改变,研究墨西哥钝口螈脊髓全切后胶质细胞的变化,对进一步探讨其脊髓切断再生机制有重要意义。目的:观察墨西哥钝口螈脊髓全切后小胶质细胞、星形胶质细胞及少突胶质细胞的变化。方法:选用成年墨西哥钝口螈,分为脊髓全切组和对照组,利用免疫组织化学法观察脊髓全切后1,3和10d的损伤脊髓及周围区cd11b标记的小胶质细胞、胶质细胞原纤维酸性蛋白标记的星形胶质细胞及髓鞘碱性蛋白标记的少突胶质细胞的变化。结果与结论:脊髓全切后短期内cd11b染色阴性;脊髓损伤后胶质细胞原纤维酸性蛋白及髓鞘碱性蛋白阳性细胞染色强度,1d组阳性细胞染色强度与对照组比较无显著差异,3及10d组阳性细胞染色强度较对照组低。墨西哥钝口螈小胶质细胞染色阴性,可能存在不同于哺乳动物的标记蛋白;脊髓全切后3及10d在损伤脊髓及周围区的胶质细胞原纤维酸性蛋白及髓鞘碱性蛋白阳性细胞染色强度较对照组低,提示钝口螈脊髓急性损伤早期未见星形胶质细胞及少突胶质细胞增生,无胶质瘢痕形成。
BACKGROUND： The spinal cord of Ambystoma mexicanum can regenerate after transection, and the number and distribution of gliacytes alter during regeneration. So it is important to observe the changes of gliacytes following spinal cord transection in Ambystoma mexicanum in order to further discuss the mechanism of spinal cord regeneration. OBJECTIVE： To observe the changes of microglias, astrocytes and oligodendrocytes following spinal cord transaction in Ambystoma mexicanum. METHODS： Adult Ambystoma mexicanum was selected and divided into spinal cord transection group and control group. Immunohistochemistry assay was performed to observe the changes in cdl 1b-labeledmicroglias, glial fibrillary acidic protein labeled astrocytes and myelin basic protein labeled oligodendrocytes in the injured region and peripheral region at 1, 3 and 10 days after spinal cord transection. RESULTS AND CONCLUSION： After transaction, the staining results of cdl lb were negative. The staining intensity results of glial fibrillary acidic protein and myelin basic protein positive cells at 3 and 10 day were lower to the control group, but showed no significant difference from the control group at I day after transection. Microglias were negative in Ambystoma mexicanum, indicating the marker proteins of microglia in salamander may be different from those in the mammals. The staining intensity results of glial fibrillary acidic protein and myelin basic protein positive cells at 3 and 10 days were lower to the control group, which suggested that astrocytes and oligodendrocytes hyperplasia were not seen and no glial scar formed in early stage of spinal cord injury in Ambystoma mexicanum.
出处
《中国组织工程研究》
CAS
CSCD
2013年第11期1993-2000,共8页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金项目(编号81271327)"BTLA修饰的未成熟树突状细胞对EAE免疫耐受作用及机制的研究"
广东省科技计划项目(编号2011B031800015)"未成熟DC诱导抗原特异性调节性T细胞及其对EAE的影响"的支持~~
