摘要
在含有NAA0.2mg/L，2.4-D 0.2mg/L的MS基础培养基中添加月光花素培养彩叶芋愈伤组织，结果显示，低浓度的月光花素能促进愈伤组织生长，高浓度则有明显的抑制作用，最适浓度为1.0～2.0mg/L；培养两个月后，添加适宜浓度的月光花素（0.2～2.0mg/L）的培养基能促进体细胞分化和植株再生，而高浓度（10.0mg/L）处理和对照均未能分化出绿苗。表明适宜浓度的月光花素在合适的生长素剂量的协同作用下，对植物愈伤组织细胞的生长和分化有较强的调节作用。
Caladium bicolor callus was cultured on the basic MS media containing calonyctin supplemented with 0.2mg/L NAA and 0.2mg/L 2,4-D. The results showed that 0.2～2.0mg/L calonyctin promoted the growth and differentiation of Caladium bicolor callus. After culturing for 2 months, the callus differentiated into plantlets on the medium 0.2～2.0mg/L calonyctin while both control and the callus treated with high concentration of calonyctin (10.0mg/L) did not regenerated plantlets. The results suggested that calonyctin with proper growth regulators has regulatory effects on the growth and differentiation of plant callus.
关键词
月光花素
彩叶芋
愈伤组织
生长
分化
调节作用
calonyctin
Caladium bicolor
callus
growth and differentiation
regulatory effect
