摘要
为探讨亚甲蓝对体外培养的人大肠癌细胞的抗肿瘤作用 ,采用直接杀伤实验、细胞生长抑制实验和细胞集落形成能力实验方法研究了亚甲蓝对体外培养的人大肠癌细胞系Lovo的作用 ,同时用透射电镜技术观察了该药对癌细胞的损伤部位 .结果显示 13 2 5 μmol/L亚甲蓝作用2 4h可杀死 79 4%的大肠癌细胞 ,5 3μmol/L亚甲蓝作用 2 4h可杀死全部大肠癌细胞 ,0 2 7μmol/L亚甲蓝即可明显抑制人结肠癌细胞的增殖 .透射电镜观察显示亚甲蓝损伤细胞的部位在线粒体 .研究结果表明 ,亚甲蓝对体外培养的人大肠癌细胞Lovo具有显著的杀伤和抑制作用 。
To study the antitumor effect of methylene blue on human colorectal carcinoma cell line Lovo in vitro , methods of direct killing and inhibition of cell growth and clonegenic survival were used and the transmission electron microscope was used to demonstrate the damage location of cancer cells.The results showed that the mortality of the human colorectal carcinoma cell line Lovo was 79 4% treated by 13 25 μmol/L of methylene blue for 24h and was 100% with 53 μmol/L of methylene blue.0 27 μmol/L of methylene blue could obviously inhibit the growth and clonegenic survival of human colorectal carcinoma cell line Lovo.The damage of cancer cells was located mainly in mitochondria. It was concluded that the methylene blue exhibited antitumor effect on human colorectal carcinoma cells in vitro ,the damage of cancer cells was located mainly in mitochondria.
出处
《沈阳药科大学学报》
CAS
CSCD
2000年第4期283-285,共3页
Journal of Shenyang Pharmaceutical University
基金
中国科学院"百人计划"资助项目
