摘要
目的 探讨应用于临床移植的人类白细胞抗原 (HLA)二类基因 (DRB/DQB)快速分型新技术。方法 采用快速DNA抽提技术 ,建立HLA DRB/DQB微量序列特异性引物聚合酶链反应(Micro PCR SSP)基因分型方法 ,对 142份供受者DNA进行HLA DRB/DQB基因分型 ,并与单克隆抗体一步法HLA血清学分型技术进行对比研究。结果 应用微量PCR SSP方法共检出 13种DRB1等位基因和 7种DQB1等位基因 ,与血清学分型结果完全相符。微量PCR SSP法仅需 1ml抗凝血 ,耗时约2h ,而且分辨率高。结论 微量PCR SSP基因分型技术具有快速、准确、省血、分辨率高等优点 ,适合在临床器官移植配型中推广应用。
Objective To develop a new technique of rapid genotyping for human leukocyte antigen (HLA) class II alleles (DRB/DQB) in clinical transplantation. Methods The micro sequence specific primer polymerase chain reaction (micro PCR SSP) genotyping technique of HLA DRB/DQB alleles based on rapid DNA extraction was developed and compared with the one step monoclonal serological HLA typing technique.Results HLA DRB/DQB genotyping was performed by micro PCR SSP in 142 DNA samples of donors and recipients, 13 DRB1 alleles and 7 DQB1 alleles were detected and there was similar results by serological technique. The micro SSP PCR technique needed only 1 ml anticoagulant blood samples spent 2 hours approximately, and had a higher resolution.Conclusion The genotyping for HLA DRB/DQB by micro PCR SSP is a rapid, accurate, blood saving and higher resolution matching technique suited for clinical application in organ transplantation tissue matching.
出处
《中华检验医学杂志》
CAS
CSCD
2000年第4期234-236,共3页
Chinese Journal of Laboratory Medicine
