摘要
[目的]为了了解黏质沙雷氏菌的抑菌机理及温度对其产生色素的影响。[方法]从土壤中分离纯化得到1株产红色素细菌,对该菌进行生理生化和16S rDNA鉴定,同时对该菌产生的红色素进行了初步的探讨。[结果]该菌为黏质沙雷氏菌(Serratia marcescens),在28℃条件下培养红色素产量最高,37℃下仍能产生色素,说明该菌株是耐高温红色素产生菌。紫外全波长扫描分析和薄板层析结果表明,其红色色素有可能是灵菌红素。[结论]该菌对霉状杆菌和镰刀菌等病原菌具有一定的抑制作用。
[Objective]The research aimed to study the antibacterial mechanism of Serratia marcescens and the effects of the temperature on the production of the red pigment.[Method] A bacterial strain producing red pigment was isolated from soil,and its morphology,biochemical,physiological characteristics and 16S rDNA sequence were investigated.[Result] The strain was identified as Serratia marcescens.The red pigment was extracted by ethyl alcohol.The maximum production was obtained at 28 ℃,still,a little pigment were synthesized at 37 ℃.This result indicated that the strain was temperature-stability.The results of ultraviolet full wavelength scanning analysis and sheet chromatography showed that the red pigment might be prodigiosin.[Conclusion] Serratia marcescens could inhibit bacterial such as fusarium and bacillus cereus mycoides.
出处
《安徽农业科学》
CAS
2013年第7期2829-2831,共3页
Journal of Anhui Agricultural Sciences
基金
国家自然科学青年基金(31100448)
博士点基金(21132-04120004)
江苏高校优势学科建设工程资助项目
关键词
红色素
黏质沙雷氏菌
鉴定
Red pigment
Serratia marcescens
Identification
