摘要
采用分光光度法测定注射用复方维生素(3)中微量草酸的方法。在氯化钾-盐酸缓冲液下,Fe3+和草酸的络合物能使Fe3+与磺基水杨酸的紫色络合物颜色变浅,测定显色反应后溶液的吸光度,试验中优化了显色反应时间、显色剂用量。结果显示,显色溶液最大吸收波长为503nm,草酸在0.006—0.036mg/mL浓度范围内,其吸光度与浓度呈良好线性关系,y=-17.552x+1.0283,r=0.9991。样品加样回收率为97.6%。本方法能准确检查注射用复方维生素(3)中微量草酸的含量。
Trace oxalic acid in compound vitamin （3） for injection was determined by spectrophotometriy. In buffer solution of potassium chloride-hydrochloric acid, Fe3＋ and oxalic acid complex can make Fe3＋ with sulfosalicylic acid violet complex of lighter color, the absorbance after color reaction was determined. Chromogenic reaction time and amount of color reagent was optimized in the tests. The results showed,the maximum absorption wavelength of color solution was 503nm. There was a good linear relationship between absorption and concentration of oxalic acid in the range of 0. 006-0. 036mg/mL. The regression equation was y=-17. 552x＋ 1. 0283,r=0. 9991. Sample recovery rate was 97.6 %. The method can accurately check the compound vitamin （3） for injection of trace oxalic acid.
出处
《光谱实验室》
CAS
2013年第3期1376-1379,共4页
Chinese Journal of Spectroscopy Laboratory
