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3种ELISA试剂盒检测片形吸虫病的效果评价 被引量：5

Effect evaluation of three ELISA kits in detection of fasciolasis
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摘要 目的评价3种人体片形吸虫病ELISA试剂盒的检测效果。方法采用本研究室大片吸虫抗原、肝片吸虫成虫可溶性抗原包被的人体片形吸虫病ELISA试剂盒(Fg ELISA和Fh ELISA)及德国DRG公司生产的人体片形吸虫IgG抗体ELISA试剂盒(DRG ELISA),分别检测26份大片吸虫患者血清、180份其他寄生虫病患者血清和26份健康人血清,评价3种检测试剂盒的检测效果。结果Fg ELISA、Fh ELISA和DRG ELISA 3种检测盒的敏感性分别为100.0%、80.8%(95%CI:65.7%～95.9%)和100.0%,特异性分别为87.9%(95%CI:83.5%～92.4%)、85.0%(95%CI:80.1%～89.9%)和83.5%(95%CI:78.4%～88.6%),约登指数分别为0.88、0.66和0.84。其中,Fg ELISA检出率(100%)明显高于Fh ELISA(80.8%)(P<0.05);Fg ELISA检测26例大片吸虫病患者血清的吸光度(A)绝对值(A/CO)为1.70,明显高于Fh ELISA的1.18(P<0.000 1)。结论Fg ELISA试剂盒检测效果较好,且成本相对较低,比Fh ELISA和DRG ELISA两法更适宜用于我国西南大片吸虫病流行区临床样本检测及大规模筛查。 Objective To evaluate the effect of 3 ELISA kits on detection of human fasciolasis. Methods Twenty-six serum samples from patients with fasciolasis, 180 serum samples from patients with other parasitic diseases as well as 26 serum samples from healthy people were detected by ELISA kits which using soluble antigen of Fasciola gigantica, Fasciola hepatica （Fg-ELISA and Fh-ELISA） as well as IgG antigen ELISA detection kits made by DRG company in Germany. The effects of the 3 kits were eval- uated. Results The sensitivities of Fg-ELISA, Fh-ELISA, and DRG-ELISA were 100.0%, 80.8% （95% CI： 65.7%-95.9%） and 100.0%, respectively; the specificities of the three were 87.9% （95% CI： 83.5%-92.4%）, 85.0% （95% CI： 80.1%-89.9%） and 83.5% （95% CI： 78.4%-88.6%）, respectively, and Youden indexes of them were 0.88, 0.66 and 0.84, respectively. The de- tection rate of Fg-ELISA （100%） was significantly higher than that of Fh-ELISA （80.8%） （P 〈0.05）. The A absolute value （A/ CO） of Fg-ELISA was 1.70, which was also significantly higher than the value of Fh-ELISA （1.18） （P 〈 0.000 1 ）. Conclusion Fg-ELISA has a good detection effect and low cost, and is more suitable than Fh-ELISA and DRG-ELISA for clinical sample tests as well as massive screening in fasciolasis endemic areas in southwest China.
作者 艾琳 陈木新 陈韶红 储言红 蔡玉春 周晓农 陈家旭
机构地区 中国疾病预防控制中心寄生虫病预防控制所
出处 《中国血吸虫病防治杂志》 CAS CSCD 2013年第2期177-181,共5页 Chinese Journal of Schistosomiasis Control
基金 中国博士后基金(2012M520353) 上海市博士后基金(12R21416500) 国家科技重大专项(2012ZX10004220 2008ZX10004 011) 国家科技支撑计划项目(2008BAI56B03)
关键词 片形吸虫病 大片吸虫 肝片吸虫 酶联免疫吸附试验 敏感性 特异性 约登指数 评价 Fasciolasis Fasciola gigantica Fasciola hepatica Enzyme-linked immunosorbent assay （ELISA） Sensitivity Specificity Youden index Evaluation
分类号 R532.29 [医药卫生—内科学]
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参考文献22

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共引文献23

同被引文献33

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中国血吸虫病防治杂志
2013年 第2期

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