摘要
以雅安扁穗牛鞭草的幼穗为外植体,研究了2,4-D浓度和不同取材部位对愈伤组织诱导的影响以及继代培养时不同浓度的2,4-D对愈伤组织增殖、分化、生根的影响。结果表明:以幼穗下部为外植体接种在MS+2,4-D 7.0 mg/L培养基中,颗粒状愈伤组织的诱导率高达100.00%;MS+2,4-D 1.0 mg/L是幼穗愈伤组织较为适宜的继代增殖培养基,在该培养基上颗粒状胚性愈伤组织的出愈率为89.44%,愈伤组织的绿苗分化率和生根率都可达100%,平均绿芽点数和生根数等均极显著高于其他处理。
In order to develop an efficient and reliable tissue culture system for warm-season forage grass Hemarthria compressa, an efficient plant regeneration system via callus induction was established using immature inflorescence as the explants. The frequency of callus induction reached 100.00% in the callus induction medium （MS） with 7 mg/L 2,4-D by taking the lower immature inflorescence. The highest embryonic callus formation reached 89.44% in the subculture medium （MS） with 1 mg/L 2,4-D. The frequency of green plant differentiation and rooting reached 100.00% of the callus after subculture on MS with 1 mg/L 2,4-D, the average number of green buds and toots significantly more than other treatments. The results would be useful for development of transgenic H. compressa plants.
出处
《广东农业科学》
CAS
CSCD
北大核心
2013年第6期22-24,F0002,共4页
Guangdong Agricultural Sciences
基金
国家科技支撑计划项目(2011BAD17800)
关键词
扁穗牛鞭草
幼穗
2
4-D
愈伤组织
继代
分化
Hemarthia compressa
immature inflorescence
2,4-D
callus
subculture
differentiation
