摘要
目的探讨CD4＋CD25＋调节性T细胞（CD4＋CD25＋Treg细胞）分泌的IL-10在梅毒血清固定现象形成中的作用。方法流式细胞仪（FCM）检测28例血清固定梅毒患者和28例健康对照外周血CD4＋CD25＋IL-10＋细胞比例及CD4＋CD25q＇reg细胞的IL-10分子数；同时用免疫磁珠细胞分选技术（MACS）分选出高纯度的CD4＋CD25＋Treg细胞后，再用实时PCR技术检测CD4＋CD25＋Treg细胞的IL-IOmRNA相对表达量。结果血清固定梅毒患者组外周血CD4＋CD25＋Treg细胞IL-10整体、蛋白和基因表达水平均比健康对照组明显增高，差异有统计学意义（均P〈0．01），但与快速血浆反应素环状卡片试验（RPR）比较，均无相关性（均P〉0．05）。结论CD4＋CD25＋Treg细胞分泌的IL-10可能参与梅毒血清固定现象形成。
Objective To investigate the possible role of intedeukin （IL）-10 secreted by peripheral CD＋ CD25＋ regulatory T （Treg） cells in the formation of seroresistance state in patients with syphilis. Methods Venous blood samples were obtained from 28 serofast patients with syphilis and 28 healthy controls. Flow cytometry （FCM） was performed to determine the proportion of CD4＋CD25＋IL-10＋ T cells in peripheral CD4＋ cells, and to quantify the protein expression of IL-10 in CD4＋CD25＋ Treg cells. Magnetic activation cell sorting （MACS） system was used to separate CD4＋CD25＋ Treg cells in high purity followed by the detection of IL-10 mRNA expression with real time PCR. Results A significant increase was observed in the proportion of CD4＋CD25＋IL-10＋ T cells in peripheral CD4＋ cells, as well as protein and mRNA expression levels of IL-10 in the serofast patients with syphilis compared with the healthy controls （all P 〈 0.01）. However, no statistical correlation was observed between rapid plasma reagent （RPR） titer and any of the above three parameters in these patients （all P 〉 0.05）. Conclusion The high level of IL-10 secreted by CD4＋CD25＋ Treg ceils may contribute to the formation of seroresistance state in patients with syphilis.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2013年第5期360-361,共2页
Chinese Journal of Dermatology
基金
国家自然科学基金（30700718）
徐州市社会发展科技计划项目（XFl0C057）
