摘要
目的研究TNF及TNFR基因多态性与中国豫北地区汉族人银屑病易感性的关系。方法采用聚合酶链反应-限制性片断长度多态性分析(PCR-RFLP)方法,检测107例中国豫北地区汉族寻常性银屑病患者(病例组)和110名健康对照者(对照组)TNFα-308,-238基因座多态性,TNFβ+252基因座多态性,以及TNFR1-329,TNFR2+676基因座多态性,用SPSS11.5软件分析这5个基因座多态性与银屑病遗传易感性的关系。结果 TNFα-238基因座、TNFβ+252和TNFR2+676基因座的基因型频率和等位基因频率分布在病例组和对照组间的差异有统计学意义(P<0.05);TNFα-308基因座和TNFR1-329基因座的基因型以及等位基因频率分布在两组间的差异无统计学意义(P>0.05)。结论中国豫北地区汉族人群中TNFα-238,TNFβ+252和TNFR2+676基因座多态性与汉族人银屑病易感性相关。
Objective To investigate the association of TNF and TNFR gene polymorphisms with the genetic suscepti-bility of psoriasis vulgaris （PV）. Methods The allele gene frequency and genotypic frequency at three Sin-gle nucleotide polymorphism （SNP） situs of TNF and TNFR in 107 PV patients and 110 heathy controls of HaM nationality in the north of Henan province were detected by polymerase chain reaction （PCR） and re-striction fragment length polymorphisms （RFLP） techniques, analyze the association of the polymorphisms at five SNP situs and the susceptibility to psoriasis vulgaris. Results The distribution of genotypes and allele of the SNP at locus TNFα-238,TNFβ＋252 and TNFR2＋676 in PV group was significantly different from that in control group（P 〈0.05 ）. There was no significant difference（P 〉 0.05 ） in the distribution of geno-type for the TNFα-308 and TNFR1-329 locus between the PV group and the health control group, and exam-ination of the allele frequencies also failed to show a difference between two groups. Conclusion These re-sults indicate that the SNP at locus TNFα-238, TNFβ ＋ 252and TNFR2 ＋ 676 are associated with PV in HaM population.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2013年第5期461-464,共4页
The Chinese Journal of Dermatovenereology
基金
新乡医学院博士启动费资助((100843)
