摘要
利用均匀设计方法优化了桃金娘SRAP反应体系。得出最佳反应体系,其总反应体积为25μL,包括2.5μL10×PCR buffer,1.0U Taq DNA聚合酶,20 ng模板DNA,0.2 mmol.L-1 dNTPs,0.3μmol.L-1引物,2.5 mmol.L-1Mg2+。采用优化的扩增体系,以Me1-Em11引物组合对8个参试种质进行SRAP扩增,扩增出的条带清晰、多态性好。所确立的体系稳定可靠,适于进行桃金娘的SRAP遗传分析。
By the method of uniform design, the reaction system for SRAP marker in Rhodomyrtus tomentosa （Ait.） Hassk. was established and optimized. The results showed that an optimal 25 μL reaction system of SRAP for R. tomentosa included 2.5 μL 10 × PCR buffer, 1.0 U Taq DNA polymerase, 20 ng template DNA, 0. 2 mmol · L^-1 dNTPs, 0.3 μmol · L^-1 primer and 2.5 mmol· L^-1 Mg^2＋. This optimum reaction system was applied to fingerprint 8 varieties of R. tomentosa accessions by Mel-Emll primers and produced clear polymorphic patterns. It showed that the optimized system could be effectively applied in the germplasm identification and genetic diversity analysis ofR. tomentosa.
出处
《植物研究》
CAS
CSCD
北大核心
2013年第3期346-350,共5页
Bulletin of Botanical Research
基金
广西自然科学基金(2010GXNSFB013025)资助
关键词
桃金娘
均匀设计
SRAP
体系优化
Rhodomyrtus tomentosa （ Ait. ） Hassk.
uniform design
SRAP
system optimization
