摘要
目的探讨多发性骨髓瘤（MM）患者骨髓间充质干细胞（BMMSC）体外生长特性和转化生长因子-β1（TGF—β1）基因表达变化及意义。方法以7例MM患者为试验组（MM组），10例单纯缺铁性贫血患者为对照组，取骨髓单个核细胞进行BMMSC体外培养，观察BMMSC体外生长形态并计数，绘制生长曲线。取BMMSC提取总RNA，反转录-聚合酶链反应（RT—PCR）法测定两组BMMSC中TGF-β1mRNA表达水平。结果MM患者BMMSC生长活性与对照组无明显差别；MM组BMMSC中TGF-β1mRNA表达水平（0．01241±0．00419）较对照组（0．00122±0．00030）升高，差异有统计学意义（t=3．218，P〈0．05）。结论MM患者BMMsc中TGF-β1高表达可能与MM发病有关。
Objective To investigate the expression and significance of transforming growth factor-β1 （TGF-β1） gene in bone marrow mesenchymal stem cell （BMMSC） derived from patients with multiple myeloma （MM）. Methods BMMSC of 7 MM patients and 10 patients with iron deficiency anemia were cultured in vitro. The morphology of BMMSC was observed and the growth curve was portrayed according to the daily results of BMMSC proliferation. Total RNA was extracted from BMMSCs and transcription of TGF-β1 gene in BMMSC was measured by reverse transcription-PCR. Results The proliferative activity of BMMSC was not significantly different between the two groups, but expression of TGF-β1 gene of BMMSC was higher in MM patients （0.01241±0.00419） than the control group （0.00122±0.00030） （t = 3.218, P 〈 0.05）. Conclusion The abnormally high expression of TGF-β1 gene in BMMSCs could contribute to the pathogenesis of MM.
出处
《白血病．淋巴瘤》
CAS
2013年第4期220-222,225,共4页
Journal of Leukemia & Lymphoma
基金
江苏省镇江市社会发展基金（WS0703）
关键词
多发性骨髓瘤
骨髓间质干细胞
转化生长因子-Β1
Multiple myeloma
Bone marrow mesenchymal stem cells
Transforming growth factor-β1
