摘要
运用水样叶绿素荧光仪(WATER-PAM)研究Cu2+胁迫下,湛江叉鞭金藻(Dicrateria zhanjiangensis)和球等鞭金藻3011(Isochrysis galbana MACC/H59)叶绿素荧光特性的变化,同时监测微藻的生长情况。结果就叶绿素荧光参数而言,湛江叉鞭金藻和球等鞭金藻3011的叶绿素荧光参数Fv/Fm,Fv/F0,Yield和ETR均随着Cu2+浓度的增大而明显降低;就生长情况而言,随着Cu2+浓度的增大,两种海洋微藻细胞密度的增长明显变缓。在24h时Cu2+对湛江叉鞭金藻的毒性大于球等鞭金藻3011,48～96h时Cu2+对球等鞭金藻3011的毒性大于湛江叉鞭金藻,Cu2+的毒性随着胁迫时间的延长而逐步增大。
The changes of the fluorescence parameters of Dicrateria zhanjiangensis and Isochrysis galbana MACC/H59 exposed to Cu^2＋ were investigated by means of the chlorophyll fluorescence technology（Water-PAM）,and the growth of these two species of marine microalgae was observed. The results showed that all fluorescence parameters （ Fv/Fm ,Fv/Fo ,Yield and ETR） of Dicrateria zhanjiangensis and Isochrysis galbana MACC/H59 decreased under Cu^2＋ stress. The growth of cell density for Dicrateria zhanjiangensis and Isochrysis galbana MACC/H59 decreased under Cu^2＋ stress, and it can be expressed as Dicrateria zhanjiangensis Isochrysis galbana MACC/H59 in the 24h while it can be expressed as Isochrysis galbana MACC/H59〉 Dicrateria zhanjiangensis during 48h to 96h. The toxicity of Dicrateria zhanjiangensis and Isochrysis galbana MACC/H59 increased with the increase of exposure time.
出处
《广西科学》
CAS
2013年第1期60-65,共6页
Guangxi Sciences
基金
广西自然科学基金北部湾重大专项(2011GXNSFE018001)
国家海洋公益性行业科研专项(201005012)
广西科学研究与技术开发计划项目(桂科合1010019-23)
广西科学院科研业务费(11YJ24HS03)资助
