摘要
目的 通过实验证明恒河猴易感和输血传播病毒 (TTV)可经肠道传播。方法 潜伏期病人粪便的抽提液 ,静脉和灌胃接种恒河猴 ,再以猴阳性粪便传代。以PCR检测猴血液和粪便、原位杂交检测活检或杀死猴的空肠和肝内的病毒。结果 静脉接种 4～ 7d、灌胃 7～ 10d发生病毒血症 ,灌胃 3～ 4d内粪便出现病毒 ,静脉接种猴血液和粪便同时出现病毒。阳性猴粪便可传代。观察 2 4周血液和粪便仍携带病毒。血清ALT不升高 ,无肝病变。肝细胞和空肠绒毛中可检出病毒。结论 恒河猴肠道感染可引起病毒血症 ,血液感染后也可由肠道排出病毒 。
Objective To test the susceptibility of the Rhesus monkey to the TT virus and to establish its transmission route. Methods Rhesus monkeys were administerted orally and intravenously with the inoculum that was prepared with feces collected from a patient at the incubation period. Passage study was done with monkey's positive feces. The blood, bile and feces were tested with polymerase chain reaction (PCR), and the liver, the jejunum tissues with in situ hybridization. Results The viremia occurred 4～7 days after intravenous inoculation and 7-10 days after oral administration . The virus was also excreted in the feces in a few days after oral infection and simultaneously with viremia after intravenous inoculation. The virus was also detected in the bile during the viremic phase. There was a prolonged carrier stat that the viremia and fecal virus excretion persisted for more than 6 months. No serum transaminase elevation was found during the infection. There were virus signals in hepatocytes in columnar epithelium and lamina propria cells of jejunum villi,but no significant pathology was demonstrated in both sites. Conclusion The liver infection of Rhesus monkey was established by either oral or parenteral virus inoculation. The virus may be released from liver into blood and intestine vial the bile or just from the gut wall into feces, and hence it may be transmitted by both routes.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2000年第3期240-243,共4页
Chinese Journal of Experimental and Clinical Virology
关键词
DNA病毒
输血
病毒
猕猴属
感染
实验
DNA, viral
Blood transfusion, viral
Macaca
Infection, experimental
