摘要
为观察碱性成纤维细胞生长因子和白细胞介素 6促进血管平滑肌细胞增殖的协同作用 ,采用大鼠主动脉贴块法培养血管平滑肌细胞 ,用 5 -溴 - 2 -脱氧尿嘧啶掺入法和细胞计数法观察碱性成纤维细胞生长因子和白细胞介素 6对平滑肌细胞增殖的影响。结果发现 ,0～ 10 .0 μg/L碱性成纤维细胞生长因子和 0～ 10 0 μg/L白细胞介素 6能促进平滑肌细胞增殖 ,且呈剂量依赖性 ,1.0 μg/L碱性成纤维细胞生长因子和 1.0 μg/L白细胞介素 6促进细胞增殖 ,且 48h内呈时效依赖性 ,作用高峰在 2 4h ,两者合用促进细胞增殖作用大于两者单用作用之和 ,呈协同作用。
Aim To observe the synergistic effect of basic fibroblast growth factor and interleukin-6 on vascular smooth muscle cells proliferation. Methods BrdU incorporation and cell counting method were adopted to observe the effects of basic fibroblast growth factor and interleukin-6 on the proliferation of rat aortic cultured smooth muscle cells. Results 0～10.0 μg/L basic fibroblast growth factor and 0～100 μg/L interleukin-6 were able to promote smooth muscle cells proliferation separately, and synergistically in combination in dose- and time-dependent manner. The pro-proliferation effect reached climax at 24 hours. Conclusion Basic fibroblast growth factor and interleukin-6 had synergistic effect on vascular smooth muscle cells proliferation.
出处
《中国动脉硬化杂志》
CAS
CSCD
2000年第3期233-236,共4页
Chinese Journal of Arteriosclerosis
关键词
BFGF
IL-6
细胞增殖
动脉粥样硬化
Fibroblast Growth Factor
Basic
Interleukin-6
Muscle
Smooth
Cell Division
Cooperative Behavior
Atherosclerosis
