摘要
目的通过三磷酸腺苷(ATP)荧光监测法和微生物培养法去评价血液透析中心环境清洁程序改进前后的清洁水平变化情况,为血液透析中心环境清洁和监测提供一个实时有效的方法。方法选择透析室5个环境位点,通过ATP荧光监测法和微生物培养法去监测5个位点的清洁水平,通过改进现有的清洁方式再次进行监测并比较两次监测结果,评价改进后的清洁程序。结果改进前5个监测位点ATP测量均值为788、310、530、600和900RLU,改进后清洁程序ATP测量均值176、130、120、220和239RLU。改进前微生物培养法监测均值为12、4、9、6和8cfu,改进后均值为2、1、1、1和2cfu。ATP荧光检测法和微生物培养法都表明有效改进清洁程序能够有效降低血液透析中心环境表面的微生物污染情况,ATP荧光检测法和常规细菌培养法监测清洁度变化趋势相同。结论有效的清洁程序能够显著降低医院环境表面的污染情况,选择ATP荧光监测法能够更快速有效地评价医院环境水平。
Objeetive Adenosine triphosphate （ATP） bioluminescence and microbiological culture methods were used for evaluation of cleaning procedure changed before or after in hemodialysis room. These provide a real-time and effective method on monitoring and evaluation of the environmental clean level of hemodialysis room. Methods Choose five sites from the hemodiaylsis room and use ATP bioluminescence and microbiological culture methods to monitor and record the clean level of those sites before and after changing clean procedure, comparison of two results and evaluation of a new clean procedure. Results The mean ofATP bioluminescence method before changing clean procedure respectively is 788.310. 530. 600 and 900RLU, after cleaning procedure changed is 176. 130. 120. 220 and 239RLU. The mean of microbiological culture method respectively is 12.4.9.6 and 8cfu, after cleaning procedure changed is 2.1.1.1 and 2cfu. The surface contaminated level of homedialysis room was significantly reduced, the monitoring results of ATP bioluminescence are similar with microbiological culture method. Conclusions Effectively surface cleaning procedure can dramatically reduced the surface contaminated condition, ATP bioluminescence is much more quick and efficient technology used in monitoring and evaluation on the nosocomial environmental clean level.
出处
《中国血液净化》
2013年第4期222-224,共3页
Chinese Journal of Blood Purification
关键词
ATP荧光监测法
微生物培养法
血液透析中心
清洁
Adenosine triphosphate （ATP） bioluminescence
microbiological culture
hemodiaylsis room
clean
