摘要
目的 为了解P16、P2 1、P2 7基因在乳腺癌中的表达状况及其与病理类型和淋巴转移的关系。方法 应用S -P免疫组织化学染色方法研究了 36例乳腺癌及 10例正常组织。结果 本组乳腺癌中P16蛋白阴性占 19.4% (7/36 ) ,P2 1蛋白阴性占 33 .3 % (12 / 36 ) ,P2 7蛋白阴性占 16 .7% (6 / 36 ) ;两种蛋白同时阴性占 11.1% (4 / 36 ) ,三种蛋白同时阴性占 2 .8% (1/ 36 )。淋巴转移组P16、P2 7蛋白阴性频率 40 % (6 / 15 )和 2 6 .7% (4 / 15 )明显高于未转移组的 4.8% (1/2 1)和 9.5 % (2 / 2 1) ,但差异无显著性 (P >0 .0 5 ) ;淋巴转移组P2 1蛋白阴性频率 6 6 .7% (10 / 15 )显著高于未转移组的 14 .3 % (3/ 2 1) (P <0 .0 5 )。结论 提示P16、P2 1、P2 7基因表达异常与乳腺癌的发生发展有关 ,可能为乳腺癌预后判断提供重要指标。
Objective To study expression of P16、P21、P27 in human breast carcinomas.Methods 36 cases of the tumor and 10 of normal tissues were stained uisng S-P immunohistochemical technique with P16、P21 and P27 monoclone antiboies.Results 19.4%(7/39),33.3%(12/36),16.7%(6/36) cases were negtive for P16、P21 and P27 protein in breast cancer respectively.The correletion rate of the two CKIs was 11.1%(4/36),and that of the three CKIs was 2.8%(1/36) in the tumor.There was obvious difference in P16 and P27 and significant difference in P21 between metastatic and non metastatic breast carcinoma (P<0.05).Conclusions The abnormal expressions of P16、P21 and P27may be related to the development of breast carcinomas. [
出处
《中国肿瘤临床与康复》
2000年第5期34-35,共2页
Chinese Journal of Clinical Oncology and Rehabilitation
