摘要
目的:本研究旨在评价吡咯烷二硫代氨基甲酸盐(PDTC)对C26癌性恶病质小鼠肌肉萎缩的调控作用。方法:采用雄性BALB/c小鼠皮下接种C26结肠癌细胞诱导癌性恶病质模型,荷瘤鼠自第7天开始每日腹腔注射生理盐水及PDTC(10、50及100 mg/kg),第16天处死小鼠后测量体重、去瘤体重及腓肠肌重量,检测肿瘤组织NF-κB活性,血清及肿瘤组织IL-6水平。结果:荷瘤对照组出现显著的去瘤体重下降及腓肠肌分解(P<0.01),血清及肿瘤组织IL-6水平显著升高(P<0.01)。PDTC能够显著抑制肿瘤组织NF-κB的激活,从而抑制了肿瘤组织IL-6的合成,其抑制程度与PDTC剂量相关,同时抑制了去瘤体重、腓肠肌的消耗,且腓肠肌重量与血清IL-6水平呈显著负相关(r=-0.87,P<0.01)。结论:PDTC能够显著抑制肿瘤组织NF-κB的激活及IL-6的合成,从而抑制癌性肌肉萎缩。
Objective： To investigate the effect of pyrrolidine dithiocarbamate （PDTC） on muscle atrophy of C26 cachectic mice. Methods： Male BALB/c mice bearing C26 adenocarcinoma were severed as cancer cachexia model. Saline and three doses of PDTC （10, 50 or 100 mg/kg） were given intraperitoneally from the 7th day after tumor inoculation to sacrifice. In the 16th day, the mice were killed, and the body weight, non-tumor body weight, gastrocnemius weight, activity of NF-κB in tumor tissue, and IL-6 levels of serum and tumor tissue were measured. Results： Significantly reduce of non-tumor body weight and gastrocnemius decomposition were observed in all tumor-bearing mice （P〈0.01）. Furthermore, Tumor-bearing caused a significant increase of IL-6 （P〈0.01） in serum and tumor tissues. Administration of PDTC significantly inhibited the NF-κB activation in tumor tissues, inhibited IL-6 synthesis of the tumor cells, and at-tenuated the non-tumor body weight and gastrocnemius muscle. There was negative correlation between IL-6 level and gastrocnemius muscle weight （r=-0.87, P〈0.01）. Conclusion： PDTC can improve muscle wasting of cancer cachexia through inhibition of NF-κB acti-vation and IL-6 synthesis.
出处
《现代生物医学进展》
CAS
2013年第6期1062-1065,共4页
Progress in Modern Biomedicine
