摘要
利用建立的pmoA、bmoX和alkB基因的定量PCR方法分别研究了油田和气田土壤中甲烷氧化菌、丁烷氧化茵和中链烷烃氧化茵的数量丰度，并以背景区土壤作为对照。pmoA和alkB的基因数量均随着采样深度的增加而降低了1—2个数量级。手工法提取油气田土壤DNA进行油气指示茵基因定量的结果显示，油田区和气田区土壤中pmoA和alkB基因丰度略高于背景区样品。气田区样品的bmoX基因丰度为2．75×10^5拷贝数／g，高于背景区和油田区土壤样品0．5-1个数量级。试剂盒提取的土壤DNA的基因定量结果表明，油田区样品pmoA和口凇基因丰度分别为3．09×10^4和2．56×10^6拷贝数／g，高于气田区和背景区土壤样品，且气田区样品bmoX基因丰度分别高于背景区和油田区样品1个数量级和0．5个数量级。结果表明3种油气指示菌的定量PCR技术的建立可为油气微生物勘探提供新的检测手段。
In this paper, the methane-, butane- and alkane-oxidizing bacteria contents in oil and gas field soil was determined by the established real-time quantitative PCR （ qPCR ） technologies targeting the pmoA, bmoX and alkB gene respectively, and the background field soils were compared as the control. The pmoA and alkB gene copies decreased by 1-2 orders of magnitude with the sampling depth increased. The gene quantification results of DNA samples extracted by manual method indicated the pmoA and alkB gene copies in oil and gas field soils were slightly higher than in background field soils. The bmoX gene copies in gas field soils was 2.75 × 10^5 copies/g, which was 0.5-1 order of magnitude higher than that in background and oil field soils. The results of DNA samples extracted by kit method showed the pmoA and alkB gene copies in oil field soils were 3.09 ×10^4 and 2.56 ×10^6 copies/g, respectively, which were higher than that of gas and background field. The bmoX gene copies in gas field soils is 1 order of magnitude higher than that of background field soils, and 0.5 order of magnitude higher than that of oil field soils. It is concluded that the established qPCR method of the three oil and gas indicating bacteria provided new detection means for microbial prospecting of oil and gas.
出处
《生物技术通报》
CAS
CSCD
北大核心
2013年第4期172-178,共7页
Biotechnology Bulletin
基金
中央高校基本科研业务费专项资金资助(JUSRP31105,JUSRP111A10)
关键词
油气微生物勘探
实时荧光定量PCR
甲烷氧化菌
烃氧化菌
Microbial prospecting of oil and gas （ MPOG ）
Real-time quantitative PCR
Methane-oxidizing bacteria
Hydrocarbon-oxidizing bacteria
