摘要
目的探讨实时超声弹性成像评价大鼠脂肪肝分级的应用价值。方法建立大鼠脂肪肝模型,分别于造模第6、8、10、12、14周随机选取大鼠进行超声弹性测量。在超声弹性成像模式下获取参照物与大鼠肝脏的应变比(B/A),然后处死大鼠,取出肝脏,进行病理切片并进行脂肪肝的分级诊断。最后将所获取的大鼠肝脏弹性数据与病理显示的脂肪浸润程度分级进行相关性分析。结果实验最终获得40只大鼠的超声弹性数据和病理结果,不同脂肪肝病理分级的B/A两两比较差异均有统计学意义(P均<0.05);B/A与其病理分级间呈相关性(r=0.88,P<0.01)。结论实时超声弹性成像为无创定量诊断脂肪肝提供了新方法,将在临床中有良好的应用前景。
Objective To explore whether the non - invasive method of ultrasound elastography can diagnose and grade hepatic steatosis by using a rat model system. Methods Fifty healthy male Wistar rats were divided into a model group （ n = 40） and a control group （ n = 10）. The model was induced by high - fat diet （20% lard）, alcohol intake （ 10% in drinking water）, and CC14 subcutaneous injections （40% in olive oil ; 0.2 ml/kg two times a week）. The control rats received normal diet, no alcohol, and olive oil injections. After weeks 6, 8, 10, 12, and 14 of the regimen, rats from each group were randomly selected and subjected to liver ultrasound elastography analysis （Hitachi HV900 Color Doppler instrument; 6 -13 MHz linear probe frequency）, first as transabdominal imaging then by fully exposed rat liver ima- ging, followed by pathological examination with hematoxylin - eosin staining. The imaging techniques were performed in triplicate for each rat and averaged. The imaging findings of controls （B） were compared to those of models （A） and used to calculate the strain ratio （B/A）. Hepatic steatosis detected by pathology was graded as percent of fatty degeneration： normal, 〈5% ; mild, 5% -33% ; moderate, 34% - 66% ; severe, 〉 66%. The correlations between B/A and pathology grades were assessed by Kruskal - Wallis and Spearman＇ s coefficient tests. Results Ten rats died during the establishment of the hepatic steatosis model system. Of the 30 surviving model rats assessed by ul- trasound and pathology, the hepatic steatosis grade was mild in 10, moderate in 12, and severe in 8; all control rats showed normal liver. The transabdominal imaging results （B/A） were significantly different between control rats （0.25±0.04） and rats with all three grades of hepatic steatosis （ vs. mild： 0.43±0.05, moderate ： 0.59 ±0.17, and severe ： 0.89±0.21 ; P 〈 0.05 ）. The B/A was positively correla- ted with the pathology grade （ r = 0. 88, P 〈 0. 001 ）. Conclusion Ultrasonic elastography effectively diagnosed hepatic steatosis and was a- ble to quantitate various pathologic grades in a non- invasive manner, suggesting its potential utility for clinical application.
出处
《临床肝胆病杂志》
CAS
2013年第4期282-285,共4页
Journal of Clinical Hepatology
基金
沈阳市科学技术计划项目(F10-205-1-07)
关键词
弹性成像技术
脂肪肝
模型
动物
elasticity imaging techniques
fatty liver
model, animal
