摘要
目的:探讨缺血预处理(IPC)对抑郁大鼠缺血/再灌注(I/R)心肌细胞凋亡及凋亡基因Fas、Fas-L表达的影响。方法:SD大鼠48只,随机分为非抑郁假手术组(Hsham),抑郁假手术组(Dsham),抑郁缺血/再灌注组(DI/R)和抑郁缺血预处理组(IPC+DI/R);采用原位末端标记法检测心肌细胞凋亡,免疫组化方法和逆转录-聚合酶链反应检测凋亡基因Fas、Fas-L的表达。结果:(1)与Hsham组、Dsham组比较,DI/R组、IPC+DI/R组的心肌细胞凋亡指数显著增加(P<0.001),Hsham与Dsham两组间比较无显著差异;与DI/R组比较,IPC+DI/R组的心肌细胞凋亡指数显著减少(P<0.001);(2)与Hsham组、Dsham组比较,DI/R组、IPC+DI/R组的Fas、Fas-L的mR-NA和蛋白表达均显著增加(P<0.001),Hsham与Dsham两组间比较无显著差异;与DI/R组比较,IPC+DI/R组Fas、Fas-L的mRNA和蛋白表达均显著减少(P<0.001)。结论:IPC能显著减少抑郁大鼠的I/R心肌细胞凋亡,其机制可能与下调Fas、Fas-L基因的表达有关。
Objective： To explore the effects of ischemic preconditioning （IPC） on myocardial apoptosis and the expression of Fas, Fas-L in depression rats that underwent myocardial ischemia/reperfu- sion （I/R）. Methods： Forty-eight SD rats were randomly divided into four groups with 12 in each group： non-depression and sham operation group （Hsham）, depression and sham operation group （Dsham）, depression and myocardial I/R group （Dr/a ）, depression, IPC and I/R group （IPC ＋ Dr/a）. The myocardial apoptosis was detected by in situ end labeling （TUNEL） method, and the expression of Fas and Fas-L were determined with immunohistochemistry and reverse transcription polymerase chain reaction （RT-PCR）. Results： （1）The myocardial apoptosis indexes in group Dr/R and group IPC ＋ Dt/R were significantly higher than those in groups Dsham and Hsham （P 〈 0.001 ）. No significant difference was found between group Hsham and group Dsham, and the myocardial apoptosis indexes in group IPC ＋ DI/R were significantly lower than those in group Dt/a （P 〈 0.001 ）. （2） The expression of Fas, Fas-L mRNA and protein in group DI/R and IPC ＋ DI/R were significantly higher than those in group Hsham and group Dsham（P 〈0.001 ）. No significant difference was observed between group Hsham and group Dsham . The expression of Fas and Fas-L mRNA and protein in group IPC ＋DI/R were significantly lower than those in group DI/ R （ P 〈 0.001 ）. Conclusions： IPC can decrease myocardial apoptosis in depression rats underwent I/R, and the mechanisms might be associated with the expression of down-regulating gene of Fas and Fas-L.
出处
《贵阳医学院学报》
CAS
2013年第2期130-133,137,共5页
Journal of Guiyang Medical College
基金
贵州省科技厅科技攻关黔科合SY子【2011】3011号
关键词
缺血预处理
再灌注损伤
抑郁障碍
细胞凋亡
基因表达
ischemic preconditioning
reperfusion injury
depressive disorder
apoptosis
gene expression
