摘要
羟基磷灰石、壳聚糖和聚乳酸通过原位生成法和溶液共混法制备三元纳米复合支架材料,将293T细胞接种于该支架材料。通过倒置荧光显微镜、扫描电镜观察材料的结构及细胞在该支架材料中黏附、生长、增殖等情况;MTT法检测细胞增殖;以及体外物理性能检测。体外实验证实该支架材料具有良好生物相容性、丰富的孔隙率、良好的力学性能等优点,对进一步开发骨组织工程支架材料应用与临床治疗具有指导意义。
Hydroxyapatite/ehitosan/poly （L-lactic acid） ternary nanocomposites scaffold was prepared by in situ method and solution blending method. In vitro 293T ceils were encapsulated into the scaffold and cultivated in three-dimensional envirnment to observe the biocompatibility of the scaffolds including cell viability, adhesion, proliferation by inverted microscope and SEM. The cell proliferation was tested by MTT method and physical performance was tested by physical method. Live/dead staining with calcein acetoxymethyl ester/Ethidium homodimer-1 （ Calcein-AM/EthD-1 ） was performed in order to determine cell viability on the scaffold. The results showed that hydroxyapatite/chitosan/poly ternary nano biocomposites scaffold display high mechanical property, hydrophilicity and good biocompatibility, suggesting the potential of this composites for applications in bone tissue engineering.
出处
《安徽医科大学学报》
CAS
北大核心
2013年第5期561-564,共4页
Acta Universitatis Medicinalis Anhui
基金
安徽省教育厅自然科学基金重大项目(编号:KJ2011ZD04
KJ2012ZD09)
国家自然科学基金(编号:81171829)
安徽省科技厅自然科学基金(编号:10040606Q36)
关键词
羟基磷灰石
壳聚糖
聚乳酸
骨组织工程
支架材料
hydroxyapatite
chitosan
poly（ L-lactic acid）
bone tissue engineering
scaffolds
