摘要
目的:探讨阿魏酸钠对佐剂性关节炎大鼠血清中TNF-α、IL-10、IL-17水平的影响,来研究阿魏酸钠的干预效果。方法:将清洁级雌性大鼠45只随机分为3组:正常对照组给予以0.9%氯化钠注射液0.1mL于大鼠足跖部,模型组和药物治疗组分别给予0.1%完全弗氏佐剂0.1mL注射大鼠足跖部,在6、12d后予以加强注射免疫。药物治疗组在造模2周后予腹腔注射阿魏酸钠,以50mg/kg每日一次,连续12d;正常对照组与模型组分别给予同等量的0.9%氯化钠注射液。于造模32d后断头处死大鼠,采集血清,酶联免疫吸附法(ELISA)测IL-15、IL-23、TGF-β浓度。结果:模型组与正常组比较,血清IL-15、IL-23水平升高,TGF-β水平降低,治疗组与模型组比较血清IL-15、IL-23表达水平明显降低,TGF-β表达水平上调。结论:阿魏酸钠可降低大鼠血清中IL-15、IL-23的水平,升高TGF-β的水平,对佐剂性关节炎有治疗具有一定的作用。
Objective. To study the effect of sodium ferulate on IL-15、IL-23、TGF-β levels in the serum of adjuvant arthritis rats, and to explore the intervention effect of sodium ferulate. Methods： 45 female Wistar rats were divided into three groups, control group received 0.1 ml doses of 0.9 % sodium chloride injec- tion in the rat paw, while model group and treatment group received 0. 1 ml doses of 0.10% Freund＇s complete adjuvant injection in the rat paw. After 6 and 12 days, immunity injections were strengthened. 14 days after modeling, treatment group received 50mg/kg doses of sodium ferulate through intraperitoneal injection once a day for 12 days;control group and model group were given equal amount of 0. 9 % sodium chloride injection. 32 days after modeling, rats were killed, and serum was collected, and then the concentrations of IL-15, IL- 23, and TGF-β were measured through enzyme--linked immunosorbent assay （ELISA）. Results. IL-15. IL -23 levels were higher and TGF-β levels were lower in adjuvant arthritis rats than those in normal control rats. The expression levels of IL-15,IL-23 in the Sodium ferulate in treated group were significantly lower than those in adjuvant arthritis rats, but the expression levels of TGF-β were increased. Conclusion. Sodium ferulate may reduce IL-15, IL-23 levels in serum, but elevated levels of TGF-β therefore, it has certain effect on adjuvant arthritis treatment.
出处
《黑龙江医药科学》
2013年第2期9-10,共2页
Heilongjiang Medicine and Pharmacy
