摘要
对花生、大豆、玉米、芝麻和丝瓜有丝分裂中期染色体进行DAPI荧光显带并比较它们DAPI带纹模式的差异。研究发现,DAPI染色能在花生、玉米染色体上显现清晰的带纹,可进行染色体识别;与花生和玉米相比,芝麻和丝瓜染色体上DAPI带纹反差较弱,能进行部分染色体识别;在大豆染色体上则没有观察到明显的带纹。这说明染色体DAPI荧光带纹模式存在明显的物种差异。
The fluorescent chromosome banding of Zea mays L., Glycine max L., Sesamum indicum L., Luffa cylindrical Roem, and A rachis hypogaea L. were revealed by DAPI staining. The results showed that there were obvious fluorescent band patterns in maize and peanut, which could facilitate chromosomes recognition. The band patters in sesame and loofah were weaker compared to maize and peanut, which could facilitate some of the chromosomes recognition. There was no clear DAPI band observed in soybean chromosomes. These results showed that there were different DAPI chromosomes banding patterns in different species.
出处
《吉林农业科学》
CSCD
北大核心
2013年第2期27-28,51,共3页
Journal of Jilin Agricultural Sciences
基金
湖北省自然科学基金项目(2010CDB04403)
湖北省教育厅科学技术研究项目(Q20101320)
