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离体大鼠心脏再灌注损伤模型中最佳再灌注时间的探讨 被引量：2

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摘要 目的:探讨离体大鼠心脏再灌注损伤模型所需的最佳再灌注时间,为进一步研究心肌缺血再灌注损伤及其保护作用提供基础。方法:Langendorff离体心脏灌流模型下,35只离体大鼠心脏随机均分成5组:①Sham组,持续灌流210min;②～⑤IR组,全心停灌30min,分别再灌注30min(IR 30min组)、60min(IR 60min组)、120min(IR 120min组)、180min(IR 180min组)。检测各时段心率血压乘积(rate-pressure product,RPP)、冠脉流出液量(coronary flow,CF)及其乳酸脱氢酶(LDH)活力,采用2,3,5-氯三苯四唑(triphenyltetrazolium chloride,TTC)染色法测量心肌梗死面积(infarct size,IS),透射电镜观察各组心肌超微结构变化。结果:与Sham组比较,RPP及CF于IR 30min组及IR60min组下降最为显著(P<0.01),LDH活力于IR 30min组及IR60min组升高最为显著(P<0.01),IS于IR 60min组开始趋于稳定;心肌超微结构于Sham组保存良好,IR 30min组其次,而于IR 60min组、IR 120min组及IR 180min组破坏均较严重。结论:在Langendorff模型下对离体大鼠心脏进行全心停灌再灌注,再灌注损伤主要发生在再灌注后60min,60min是相对较合适的再灌注时间。
作者 黎庆捷 文伟明 钟国强 涂荣会 何艳 李烁 蒙滋滋
机构地区 广西医科大学第一附属医院老年心内科
出处 《实用医学杂志》 CAS 北大核心 2013年第8期1237-1239,共3页 The Journal of Practical Medicine
基金 国家自然科学基金资助项目(编号:30960131) 广西自然科学基金资助项目(编号:2012GXNSFAA053154)
关键词 离体心脏灌流 再灌注损伤 再灌注时间
分类号 R542.22 [医药卫生—心血管疾病]
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参考文献9

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共引文献18

同被引文献27

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实用医学杂志
2013年 第8期

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