摘要
分析了沙冬青(Ammopiptanthus mongolicus)锌指蛋白基因AmZFPG在非生物胁迫下的表达特性,结果显示AmZFPG受低温、干旱、高盐胁迫诱导表达,表明该蛋白参与多种胁迫相关的信号转导和应答反应。为进一步探索AmZFPG的功能,构建了真核表达载体AmZFPG-pCAMBIA2300,将该基因转入烟草(Nicotiana tabacum L.),对转基因烟草T1代进行非生物胁迫分析,结果显示,转基因烟草的耐寒性、耐旱性、耐盐性均获得了提高。
The characterization of a novel gene designated AmZFPG from Ammopiptanthus mongolicus was described here. AmZFPG encodes a zinc-finger protein that is induced after different types of stresses, namely cold, desiccation, and salt. Overexpression of the gene in transgenic tobacco conferred tolerance to cold, dehydration, and salt stress at the seed-germination/seedling stage as reflected by the percentage of germination, the fresh weight of seedlings, and their growth pattern. Thus, AmZFPG seems to be an important determinant of stress response.
出处
《园艺学报》
CAS
CSCD
北大核心
2013年第4期713-723,共11页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31270737,30671476)
北京市自然科学基金项目(6112016)
