摘要
目的观察官入菌(米雅 BM)对实验性急性坏死性胰腺炎(ANP)肠道细菌移居的影响。方法 SD 大鼠随机分成三组,对照组、ANP 组和 ANP 米雅BM 处理组,ANP 大鼠模型制备采用胰胆管内注射5%牛磺胆酸钠溶液,观察胰腺及肠道病理形态学改变,血清淀粉酶和脂肪酶活性变化,血清内毒素水平,脏器细菌培养,肠道细菌菌群变化及肠道 sIgA 含量。结果 ANP 组血清淀粉酶、脂肪酶活性为(9091±1901)nkat·L^(-1),(1677±391)nkat·L^(-1),较对照组[(2588±291)nkat·L～(-1),(121±30)nkat·L^(-1)]明显增高,P<0.05;米雅 BM 处理组血清淀粉酶、脂肪酶活性分别为(5528±1195)nkat·L^(-1),(495±129)nkat·L～(-1),较 ANP 组显著下降,P<0.05.米雅 BM 处理组血清内毒素水平为(0.039±O.011)EU·L～(-1),较 ANP 组[(0.067±0.012)Eu·L^(-1)]显著降低,米雅 BM 能显著纠正业已紊乱的肠道菌群,促进肠道有益菌生长,抑制过度繁殖的大肠杆菌等有害菌生长,显著提高肠粘膜双歧杆菌/大肠杆菌(1.96±0.21 vs 1.53±0.31),从而控制肠道细菌移居,脏器细菌培养阳性率显著下降(37.5% vs 80%),肠道 sIgA 含量在 ANP组为(42±18)pg/g,而米雅 BM 处理组[(20±4)pg/g]低于 ANP组,米雅 BM 能显著减轻胰腺及肠道粘膜病理形态损害。结论米雅 BM 可通过调节肠道微生态紊乱,保护肠粘膜屏障,控制 ANP 状态下肠道细菌和毒素移居,从而改善 ANP 肠道及胰腺病理损害。
AIM To evaluate the effects of clostridium butyricum on bacterial translocation in rats with acute necrotizing pancreatitis(ANP). METHODS Twenty-seven SD rats were assigned to three groups randomly,including sham-operation,ANP without treatment and ANP treated with clostridium butyricum. ANP model was made by injection of 5% sodium tarucholate into the bilio-pancreatic duct.Pathological changes in pancreas and ileum were studied.Serum lipase and amylase activities and serum endotoxin were determined.Bacterial culture in pancreas,liver,spleen and mesenteric lymph nodes(MLN)were made.Changes in intestinal bacterial flora and slgA were investigated. RESULTS Serum amylase and lipase activities were decreased in clostridium butyricum treated rats(5528± 1195)nkat.L^(-1),(495±129)nkat.L^(-1) compared with ANP group(9091±1901)nkat.L^(-1),(1677±391)nkat.L^(-1) (P<0.05).Serum endotoxin level was increased in ANP group(0.067±0.012)EU·L^(-1),but decreased in clostridium butyricum treated rats(0.039±0.011)EU· L^(-1) (P<0.05).Clostridium butyricum could regulate the imbalance between intestinal anaerobic bacteria and aerobic bacteria in ANP rats.The B/E ratio was 1.53± 0.31 in ANP group,but increased to 1.96±0.21 in clostridium butyricum group.The incidence of bacterial translocation in ANP(80%)was reduced by clostridium butyricum treatment(37.5%).Intestinal slgA was increased in ANP,but decreased in clostridium butyricum treatment,Pathological changes in pancreas and ileum were ameliorated by administration of clostridium butyricum. CONCLUSION Clostridium butyricum could improve the outcome of rats with ANP,Owing to its action of inhibiting intestinal bacterial translocation.
出处
《世界华人消化杂志》
CAS
2000年第8期883-886,共4页
World Chinese Journal of Digestology
基金
上海市科学技术委员会科技启明星计划资助课题
No.99QB14009
关键词
胰腺炎
急性
肠道细菌移居
宫入菌
Subject headings Pancreatitis/acute
intestinal bacterial translocation
clostridium butyricum
