摘要
根据ACC合酶高度保守区氨基酸序列设计两种兼并引物。通过RTPCR,克隆了香蕉果肉ACC合酶1693bp的cDNA片段。再根据其序列测定结果进行5′RACE(RapidamplificationofcDNAends)。最终确定香蕉果肉中ACC合酶的mRNA全长为1752个核苷酸。其中5′非翻译区74个核苷酸,编码区1461个核苷酸,3′非翻译区217个核苷酸,编码产物为486个氨基酸。通过Northern杂交分析。
A cDNA encoding ACC synthase in banana pulp was amplified by RT PCR and cloned in E.coli. The 5′terminal region of the ACC synthase transcript was determined by using 5′ RACE procedure.The results showed that the ACC synthase cDNA in banana pulp is 1752 bp in length including 74bp of 5′untranslated region,1461 bp of coding region which encodes a polypeptide of 486 amino acides and 217 bp of 3′untranslated region.The Northern blot analysis indicates that the ACC synthase mRNA is specifically transcripted in banana fruits.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第2期134-136,共3页
Chinese Journal of Biotechnology
基金
中国科学院"九五"重大项目资助课题
