摘要
要在一种宿主表达系统中成功表达外源蛋白并获得较高产量 ,必须要较为全面地了解影响其表达的诸多因素。影响外源基因在巴氏毕赤酵母中表达的因素主要包括 :外源基因的特性、表达框的染色体整合位点和方式、宿主菌的甲醇利用表型、基因剂量、分泌信号、产物稳定性和翻译后修饰等。本文就这些因素进行分析 ,并提出一定的对策和建议。
Numerous factors that influence heterologous protein production in a certain host system must be observed to gain successful gene expression and even with high yields. In Pichia pastoris (Pp) they includes: properties of heterologous gene, site and method of integration of the expression cassette, methanol utilization phenotype of the host, gene dosage, nature of secretion signal, stability of the product and post?translation modification. A fuller analysis, together with some corresponding strategies and/or suggestions is given below.
出处
《生物技术通讯》
CAS
2000年第2期132-134,140,共4页
Letters in Biotechnology
关键词
巴氏毕赤酵母
外源基因表达
影响因素
Pichia pastoris
heterologous gene expression
influential factors
