摘要
通过正交设计试验，优化了黑曲霉（Ａｓｐｅｒｇｉｌｌｕｓ ｎｉｇｅｒ ｍ１２）产胞外木聚糖酶的液体发酵条件，合适的产酶培养基含麦草粉３ｇ，（ＮＨ４）２Ｓ０４０．３ｇ，ＫＨ２Ｐ０４０．１ｇ，ＭｇＳＯ４·７Ｈ２Ｏ０．０５ｇ，ＮａＣｌ０．０３ｇ，Ｔｗｅｅｎ８００．３ｇ，酵母膏０．１ｇ，微量元素（Ｂ、Ｚｎ、Ｍｎ、Ｆｅ、Ｍｏ）定容１Ｌ；接种量为１．０×１０＾７个孢子／５０ｍＬ培养基，２８℃，１２０ｒ／ｍｉｎ，
Through orthogonal experiment, the optimal condition was given for producing extracellular xylanases of Aspergillus niger ml2. The suitable medium contained 3% wheat-straw powder, 0.3%(NH_4)_2SO_4, 0.1% KH_2OP_4, 0.05% MgSO_4·7H_2O,0.03% NaCl,0.3% Tween 80, 0.1% yeast extract, and trace elements (B, Zn, Mn, Fe and Mo). 1.0 × 10_7 spores were inoculated into 50mL medium in 250mL Erlenmeyer Flask in a water rocker (120r/min,28℃) which stirred for 5.5 days. The xylanase activity of culture filtrate of ml2 was 34.47u/mL.
出处
《微生物学通报》
CAS
CSCD
北大核心
2000年第2期108-112,共5页
Microbiology China
基金
山东省科委资助!No.971164805
关键词
木聚糖酶
黑曲霉m12
发酵
正交试验
Xylanases, Aspergillus niger ml2, Fermentation, Orthogonal experiment
