摘要
对进口 X祖代鸡 B系和 D系共 1 43羽异性公鸡的 K座位羽速型作了 K座位隐性测交 ,杂合子占 41 .96% ,慢羽纯合子占 35.66% ,另有 9羽和 2羽公鸡分别因后裔数少于 7羽或是后裔快慢羽鸡比例严重偏离 1∶ 1而被判为无效 .对 8羽进口祖代 D系异性公鸡的PCR- RFLP分析的结果表明 ,6羽公鸡为 K座位显性纯合子 (仅检到 1 450 bp DNA片段 ) ,2羽为杂合子 (检到 1 450 bp、1 0 68bp和 382 bp共 3条带 ) .通过 K座位的检测 ,降低了进口祖代鸡中的非同质个体比例 ,提高了复制育种的鸡群整齐程度以及父母代种鸡按羽速型自别雌雄的正确率 ,是一个实用有效的方法 .全面的遗传质量监测还应考虑到更多的基因座位乃至对整个基因组的遗传监测 .
A total of 143 cocks in lines B and D from an imported grant parent breeding stock was genotyped by recessive backcross. The result indicated that heterozygotes (Kk +) and dominant homozygotes (KK) made up 41.96% and 35.66% respectively in this population. PCR RFLP detection showed that 6 cocks of 8 imported line D were dominant homozygotes, which were characterized by the intact PCR fragment of 1 450 bp after digestion with HaeⅢ. However, 3 fragments (1 450 bp, 1 068 bp and 382 bp) were discovered in the rest two cocks, suggesting that they were heterozygous at locus K. Increased homogeneity at locus K were associated with improved homogeneity in progeny derived from so called “copy breeding” of imported lines, and improved rate of feathering sexing in parental stocks. It was also suggested that mult loci, or even the whole genome, should be involved for a systematic genetic evaluation in a more complicated situation.
出处
《生命科学研究》
CAS
CSCD
2000年第3期250-254,共5页
Life Science Research
基金
湖南省科委攻关计划
湖南省自然科学基金资助项目!(91 -0 7-0 0 1 )
关键词
遗传同质性
蛋鸡
隐性测交
羽速基因
异性个体
genetic evaluation
layers
recessive backcross
PCR RFLP
feathering alleles
