摘要
目的观察汉族与维吾尔族肾癌患者之间血清蛋白表达谱的变化，建立维吾尔族、汉族不同民族肾癌血清诊断模型并筛查2个不同民族肾癌患者之间的差异蛋白。方法采用表面增强激光解吸离子化飞行时间质谱技术和弱阳离子交换蛋白芯片（CMIO）检测血清标本，对新疆地区24例维吾尔族肾癌患者与24例维吾尔族健康对照血清蛋白表达谱进行检测分析；对36例汉族肾癌患者与36例汉族健康对照血清蛋白质指纹图谱进行检测分析。结果维吾尔族肾癌组与维吾尔族健康对照组组间得到差异有统计学意义的6个蛋白质峰，分别为5935、5945、5911、13766、13965、6887，建立的诊断模型的敏感度为100．00％（24／24），特异度为91．67％（22／24）；汉族肾癌组与汉族健康对照组组间得到的差异有统计学意义的4个蛋白质峰分别为5937、5345、5947、5912，建立的诊断模型的敏感度为’91．67％（33／36），特异度为94．44％（34／36）；维吾尔族与汉族两个不同民族肾癌之间的血清蛋白质指纹图谱差异有统计学意义（P〈0．05）。质荷比为6887、13766、13965的3个蛋白峰为维吾尔族肾癌诊断模型所独有，而质荷比为5345的蛋白峰为汉族肾癌诊断模型所独有。结论新疆地区维吾尔族。肾癌与汉族肾癌患者之间血清蛋白质指纹图谱差异有统计学意义，质荷比为6887、13766、13965、5345的蛋白峰可能是维汉不同民族肾癌的差异蛋白。其中M／Z为6887、13766、13965蛋白峰对应的可能蛋白质分别为为角蛋白、甲状腺素转运蛋白及干扰素诱导的跨膜蛋白。1。
Objective To observe serum protein change between Uygur with renal cell cancer （RCC） and Han with RCC and to construct different ethnic（including Han and Uygur） diagnostic model of RCC in XinJiang and screen serum differentially expressed protein between Han with renal cancer and Uygur with renal cancer in Xinjiang;Methods To use weak cation exchange and hydrophobic surface protein chip （CMI0） and Surface-enhanced laser desorption ionization time of flight mass spectrometry （SELDI-TOF-MS） to detect the serum protein. To detected serum specimens from patients （36 Han and 24 Uygnr）with RCC and 60 healthy controls so as to construct different ethnic RCC diagnostic model and screen out disparate serum proteins between Hart with RCC and Uygur with RCC. Results Obvious disparity existed between healthy controls and RCC patients in Uygur. Diagnostic model was composed and established by six proteins （5935,5945,5911,13 766,13 965,6887）. The specificity and sensitivity were 91.67% （22/24） and 100. 00% （24/24） ; Obvious disparity existed between healthy controls and RCC patients in Hart. Total were four proteins peaks （5937,5345,5947,5912）, and diagnostic model composed and established. The specificity and sensitivity were 94. 44% （34/36） and 94.67% （33/36） ; disparity existed between Uygur RCC patients and Han RCC patients in terms of serum protein finger prints. Three proteins peaks M/Z 6887,13 766,13 965 existed diagnostic model of Uygnr patients group while lacked in Han patients group, meanwhile, M/Z 5345 existed diagnostic model of Han patients group while lacked in Uygnr patients group. Conclusion It has difference for protein finger prints between Han with RCC and Uygnr with RCC in Xin- jing. M/Z 6887,13 766,13 965,5345 may be differential markers between Uygnr with RCC and Han with RCC and M/Z 6887,13 766,13 965 may be Keratin, transthyretin and interferon-induced.transmembrane protein-1.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第4期845-847,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（81060210）
