摘要
研究了离子强度、缓冲液 p H、慢离子浓度、凝胶浓度等因素对小麦蛋白质 APAGE分离效果的影响 ,采用 L1 6 ( 41× 2 1 2 )正交试验进行了条件优化 ,所建立的甲酸盐 APAGE方法 ,具有分辩率高、重复性好的特点 ,可通过改变凝胶浓度实现不同蛋白的分离研究。因而此法在小麦蛋白分离、遗传研究。
The separation effect of wheat protein APAGE was researched by analysing some influence factors such as ion strength, gel buffer pH, slow ion conentration, gel concentration etc, and the optimum condition was done by L 16 (4 1×2 12 ) orthogonal experiment. The formate APAGE method of wheat protein established had the porperties of high resolving power and stability. The separation reasearch of wheat protein could be realized through changing the gel concentration. The method was applicable to wheat protein separation, genetic studies and variety identification.
出处
《麦类作物学报》
CAS
CSCD
2000年第4期55-58,共4页
Journal of Triticeae Crops
