摘要
目的探讨自发性腹膜炎快速诊断的方法。方法利用细菌特有16SrRNA基因序列片段设计引物,分别通过单引物和双引物扩增标准菌种样品,通过PCR-SSCP分析常见致病菌的单链构象差异。结果建立了基于16SrRNA基因序列的自发性腹膜炎7种常见致病菌的PCR-SSCP图谱。结论 PCR-SSCP技术可快速检测及鉴定自发性腹膜炎常见致病菌。
Objective To investigate on the rapid diagnosis method of spontaneous bacterial peritonitis （SBP）. Methods Primers were designed according to the 16S rRNA gene fragment sequence of bacteria. The DNA templates isolated from the standard strains were amplified using one pair and double pairs primers, then the single strand confornation polymorphism of these bacterial was analyzed by PCR-SSCP. Results PCR-SSCP diagram of seven common bacterial species in SBP patients was established successfully. Conclusions PCR- SSCP diagram could detect and identify pathogenic bacteria of SBP rapidly and conveniently. [ Key words ] Polymerase chain reaction; Single-strand conformation polymorphism; Bacteria; Spontaneous bacterial peritontis
出处
《中华实验和临床感染病杂志（电子版）》
CAS
2013年第1期9-11,共3页
Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)
基金
首都医学发展科研基金(No.2007-3054)
关键词
聚合酶链反应
单链构象多态性
细菌
自发性腹膜炎
Polymerase chain reaction
Single-strand conformation polymorphism
Bacteria
Spontaneous bacterial peritontis
