摘要
利用粘粒载体 pCOS5构建了国内分离的O139霍乱弧菌的基因组文库 ,并从文库中筛选获得可以表达O139霍乱弧菌脂多糖的重组克隆株E .coliJM10 9(pMG310 )。重组粘粒 pMG310经酶切分析 ,所克隆的外源DNA片段大小为 37kb。实验证明 :重组克隆株E .coliJM10 9(pMG310 )
The gene cluster that determines the biosynthesis of the lipopolysaccharide(LPS) of V.cholerae O139 was cloned and expressed in E.coli JM109 by constructing genomic library of Vibrio cholerae O139. A colony that expressed LPS of Vibrio cholerae O139 was detected and designated E.coli JM109(pMG310). Restriction analysis of the recombinant cosmid pMG310 demonstrated that the DNA fragment was 52kb. The LPS gene of Vibrio cholerae O139 that was linked to Vector pCOS5 was about 37 kb. Immunochemical assays demonstrated that LPS expressed by E.coli JM109(pMG310) had the same immunogenicity and reactogenicity as LPS of Vibrio cholerae O139.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第6期699-702,共4页
Chinese Journal of Biotechnology
基金
国家高技术研究发展与计划项目资助!( 10 2 0 7 0 3 0 2
