摘要
以超声波辅助法提取黄山贡菊的总黄酮,以还原力、金属离子螯合性和对DPPH自由基、亚硝酸钠的清除能力为指标评价黄山贡菊总黄酮的抗氧化活性.结果表明,总黄酮还原力的IC50为0.171mg/mL,金属离子螯合性的IC50为1.529mg/mL,总黄酮对DPPH.和亚硝酸钠清除作用的IC50分别为0.323、0.700mg/mL.黄山贡菊总黄酮具有较强体外抗氧化活性,活性与浓度存在一定的量效关系.
The total flavonoids from Dendranthema morifolium Tzvel. cv. Gongju was extracted with the assistance of ultrasound. The antioxidant activities of total flavonoids was evalua- ted by reducing power, metal chelating ability, and scavenging capacity against DPPH ·, sodium nitrite. The results showed that the total flavonoids had reducing power with IC50 value of 0. 171 mg/mL and metal chelating ability with IGs0 value of 1. 529 mg/mL. At the same time, the total flavonoids exhibited scavenging capacity against DPPH ·, and sodium nitrite with ICs0 values of 0. 323.0. 700 mg/mL, respectively. The total flavonoids possessed good antioxidant activities in vitro in a concentration-dependent fashion.
出处
《华中师范大学学报（自然科学版）》
CAS
北大核心
2013年第2期218-220,共3页
Journal of Central China Normal University：Natural Sciences
基金
中国博士后科学基金项目(2011M500898)
黄山学院博士启动基金项目(2012xkjq002)
黄山学院大学生创新基金项目(2012xdkj016)
关键词
黄山贡菊
总黄酮
抗氧化活性
Dendranthema morifolium Tzvel. cv. Gongju
total flavonoids
antioxidant
