摘要
目的:研究"益气活血解毒方"对肾癌移植瘤的作用机制。方法:①将体外培养的小鼠Renca肾癌细胞分为对照组、氟尿嘧啶组和益气活血解毒方组。分别加入RPMI 1640培养液、氟尿嘧啶(4 mg/mL)、益气活血解毒方(300μg/mL),48 h后,四甲基偶氮唑蓝(MTT)法测定细胞增殖。②将36只荷瘤小鼠随机分为生理盐水组、氟尿嘧啶组和益气活血解毒方组,每组12只,分别于移植瘤内多点注射生理盐水、氟尿嘧啶、益气活血解毒方治疗28 d。对抑瘤率、PTEN、Survivin、VEGF和P-gp在小鼠肾癌移植瘤中的表达进行分析。结果:①在体外实验中,益气活血解毒方能明显抑制肿瘤细胞增殖,抑制率为43.02%。②在体内实验中,益气活血解毒方组抑瘤率为29.48%,氟嘧啶组抑瘤率为2.84%。益气活血解毒方组Survivin、VEGF、P-gp和PTEN蛋白阳性表达率分别为:25.0%、41.7、41.7%、91.7%,氟嘧啶组四种蛋白分别阳性表达率为41.7%、58.3%、83.3%、58.3%,生理盐水组四种蛋白分别阳性表达率为100%、91.7%、75.0%、16.7%,差异有统计学意义。结论:益气活血解毒方在体内外均有较强的抑制肾癌的作用,其抗肿瘤作用机制是多靶点、多通道的。
Objective：To observe the effect of Yiqi Huoxue Jiedu Formula on mice renal carcinoma. Methods：（1）The cell line of Renca mice renal cell carcinoma was treated with Yiqi Huoxue Jiedu Formula,5 - FU and RPMI 1640 culture medium, respectively. MTT assay was used to evaluate the cytotoxic effects of Yiqi Huoxue Jiedu Formula on Renca cells. （2）The model of RCC was established in mice. Comparative analysis between control and experimental groups on the sur- vival of mice and the growth of tumor. Expressions of PTEN, Survivin, VEGF and P - gp were evaluated by the method of immunohistochemistry. Results：Yiqi Huoxue Jiedu Formula inhibited the growth of Renca ceils significantly in vivo. The average tumor weight was far lighter than that in control group. Yiqi Huoxue Jiedu Formula significantly increased the ex- pression of PTEN in tumor tissue. And Yiqi Huoxue Jiedu Formula significantly decreased the expressions of Survivin, VEGF and P-gp in tumor tissue. Conclusion：Yiqi Huoxue Jiedu Formola can induce the multiple anti -RCC effect.
出处
《中华中医药学刊》
CAS
2013年第4期804-807,I0002,共5页
Chinese Archives of Traditional Chinese Medicine
基金
山西省科技攻关计划资助项目(20090311066)
关键词
益气活血解毒方
肾癌
小鼠
Yiqi Huoxue Jiedu Formula
renal cell carcinoma
mice
