摘要
采用孙女设计法对德国奶牛的3个数量性状——产奶量、乳脂产量及乳蛋白产量进行了数量性状基因位点的定位研究。实验共有20个父系半同胞家系的1 130头种公牛，共测定了30对染色体上的229个微卫星，表型性状的育种值由孙代母牛估计得到。连锁分析采用多重遗传标记回归法，临界F值由置换实验法计算得到。统计检验发现在某些染色体上存在着一些显著的QTL’s区域，特别是14号染色体上某一区域对所有3个产奶性状都有极显著的影响。进一步证实了Coppieters和Ron等人的实验结果。
A total of 1 130 bulls belonging to 20 half-sib families of German dairy cattle were genotyped for 229 microsatellite markers on 30 chromosomes. The data were used in an attempt to map quantitative trait loci applying regression as multiple-marker regression. For association analysis with a granddaughter design, the estimated breeding values for 3 milk traits were used: milk production, fat production and protein production. The empirical values of significance thresholds were determined by using a permutation test on the experimental data. Several significant QTLs were found on some chromosomes, especially on the chromosome 14. The results give a strong support for the experiments of Coppieters et al. and Ron et al.
基金
德国科学技术部及德国奶牛育种协会项目
关键词
数量性状基因位点
微卫星
孙女设计法
奶牛
QTL
multiple-marker regression
microsatellite
granddaughter design
permutation test
