摘要
目的 探讨白色念珠菌蛋白酶活动与其毒力的关系。方法 采用牛奶平板法检测 5 4株白色念珠菌蛋白酶的分泌能力 ,然后选择 6株菌分别经摇瓶培养测定其蛋白酶活力 ,并以静脉内注射方式感染小鼠进行毒力试验 ,以小鼠平均生存时间来评价菌株毒力。结果 5 4株白色念珠菌全部能分泌蛋白酶 ,检出率为 10 0 %。动物试验表明蛋白酶活力愈高的菌株 ,相应小鼠平均生存时间愈短 ;蛋白酶活力与菌株毒力直接正相关 (r=0 .934,P〈0 .0 1)。结论 蛋白酶是白色念珠菌重要的毒力因子 。
Objective to study the relationship between Candida Albicans proteinase activity and virulence in mice.Methods proteinase secretion of the 54 C. albicans strains was detected on milk agar plate.Six strains were selected and their proteolytic activity was determined after incubation on ashaker.The virulence was espressed as the mean sruvival time of mice.Results All of 54 strains tested secreted proteinase indicating that the detection rate was 100%.Animal experiment showed that the higher proteinase activity was associated with the shorter average survival time of mice.There was a directly positive correlation between proteinase activity and virulence( r =0.934, P 〈0.01).Conlusions These results indicate that extracelluar proteinase is a putative virulence factor for C.Albicans .Proteinase activity can be used as the direct parameter for virulence of C.Albicans.
出处
《中国微生态学杂志》
CAS
CSCD
2000年第4期215-216,220,共3页
Chinese Journal of Microecology
