摘要
利用ＲＡＰＤ技术对香菇６个双单杂交菌株及其两个双核体基因组ＤＮＡ进行了检测，结果显示：１～３号杂交菌株之间的相似系数为０．８９３～０．９６２，它们与其亲本Ｓ之间的相似系数为０．８４２～０．８５９；４～６号杂交菌株之间的相似系数０．８５７～０．９２５，与其亲本ＳＳＯ１之间相似系数为０．７０８～０．９０２表明杂交菌株与其双核体亲本基因组具有较大差异，杂交菌株之间存在着不同程度差异，６个杂交菌株是真正的杂交后代，聚类分析树状图直观地表明了菌株间的遗传相关性，认为菌株间ＤＮＡ的相似系数值可以作为杂交育种选择亲本的辅助的遗传标记。
In the present paper, RAPD techniques were used to detect genomes DNA of 6 dimon mating hybrids and their parents. The results showed that the similarity coefficients varied within 0.842-0.859 among the hybrids from 1 to 3 and within 0.893-0.962 between they and their parent S; The similarity coefficients among the hybrids from 4 to 6 and between they and their parent SSO1 were respectively 0.857-0.925 and 0.708-0.902, suggesting obvious difference at genome DNA level of hybrids and their dikaryonic parent genetic variation at different DNA level among the hybrids and 6 strains of hybrids that are true. Dendrogram of cluster analysts on DNA similarity coefficient of strains showed directly genetic relationships among them and the value of similaity coefficient between strains is taken for a supplementary genetic marker of selecting parents and mating breeding.
出处
《微生物学通报》
CAS
CSCD
北大核心
2000年第4期283-286,共4页
Microbiology China
关键词
香菇
双单杂交
RAPD
相似系数值
聚类分析
Lentinula edodes, Di-mon mating, RAPD, Value of similarity coefficient Cluster analysis
