摘要
对处于磷酸盐饥饿条件下的番茄幼苗根部质膜以及去除质膜的其他膜部分的蛋白质含量及组分的变化进行了检测。结果显示 ,磷酸盐饥饿第 7d时 ,受胁迫苗根部质膜及去除质膜的其他膜蛋白质含量与各自的对照相当。而SDS -PAGE结果表明 ,磷酸盐饥饿第 7d时受胁迫苗根部质膜蛋白质中出现 4条对照中所没有的新的多肽 (分子量分别为 34kD ,36kD ,4 6kD和 4 9kD)。该结果经浓度梯度电泳得到进一步的证实。本文推测在受胁迫苗根部质膜中出现的这些新的多肽可能是磷酸盐饥饿诱导蛋白并作为磷酸盐饥饿救援系统的组分。但在去除质膜的其他膜蛋白质中未看到胁迫处理与对照之间多肽表达的明显的差别。
The changes of protein components of plasma membranes and plasma-membrane-depleted vesicles from the roots of tomato seedlings under phosphate starvation were studied in this paper. Firstly, the results revealed that, under phosphate starvation for 7 days, the contents of proteins of plasma membranes and plasma-membrane-depleted vesicles from the roots of tomato seedlings approximated to those of their unstressed controls respectively. Secondly, the results from SDS-PAGE of proteins showed that there were four novel polypeptides (mw: 34 kD, 36 kD, 46 kD, 49 kD) which existed in the plasma membranes from the roots of tomato seedlings under phosphate starvation for 7 days but not in those of controls. The above-mentioned results of SDS-PAGE were further confirmed by CG-PAGE. It was supposed that these new polypeptides might be specifically induced by phosphate starvation and as the components of phosphate starvation rescue systems. However, the results in this study did not find the existance of differential expression of proteins of plasma-membrane-depleted vesicles in the stressed seedlings and their controls.
出处
《云南植物研究》
CSCD
2000年第3期322-330,共9页
Acta Botanica Yunnanica
基金
ThisworkwassupportedbytheNationalNaturalScienceFoundationofChina
TheexperimentswerecarriedoutinShanghaiInstituteofPlantPhysio
关键词
番茄
磷酸盐饥饿
救援系统
诱导蛋白
Tomato( Lycopersicon esculentum )
Phosphate starvation
Rescue system
Inducible proteins
