摘要
目的探讨构建C57BL/6小鼠FBL-3微小残留白血病(MRL)模型的方法。方法将5×106个FBL-3红白血病细胞经尾静脉接种至C57BL/6小鼠,接种后第3天经尾静脉注射不同剂量环磷酰胺(CTX),观察小鼠生存时间与药物剂量间的关系;根据白血病小鼠生存时间与CTX剂量和与接种FBL-3细胞数量的关系,确定构建小鼠MRL模型所需的化疗药物剂量。结果 C57BL/6白血病小鼠对CTX敏感,随CTX剂量增加白血病小鼠的生存时间逐渐延长,药物剂量与生存时间有较好的回归关系。移植生物试验显示,接种500个FBL-3细胞的小鼠生存时间与接受100 mg·kg-1CTX化疗白血病小鼠相当。结论每只C57BL/6小鼠经尾静脉接种5×106个FBL-3细胞3 d后给予100 mg·kg-1CTX化疗可以建立MRL动物模型。
Objective To establish FBL-3 minimal residual leukemia （MRL） model in C57BIZ6 mice. Meth- ods 5 × 10^6 FBL-3 cells were inoculated intravenously into C57BL/6 mice and the relationship of the amount of inoculated cells and the survival time of mouse was investigated. On the third day, cyclophosphamide （CTX） of different dose was infused into these mice through vena caudalis and the relationship of the dose of CTX and the survival time of mouse was investigated. Results of the above two steps were combined to decide the close of CTX in construction of minimal residual leukemia model of FBL-3 cells in mice. Results The survival time increased following the increase of the doses of CTX. The model of leukemia was sensitive to CTX and the time of survival was related to the dose of CTX received. The results of biological transplantation experiments indicated that the survival time of the inoculated mouse treated with CTX at 100 mg · kg-1 was equal to that of the mouse inoculated with 500 FBL-3 cells without treatment. Conclusion MRL Model could be established by C57BL/6 mice intravenously inoculated 5 × 10^6 FBL-3 cell and chemotherapy by 100 mg·kg-1 CTX after inoculation the third day.
出处
《肿瘤基础与临床》
2013年第2期97-99,共3页
journal of basic and clinical oncology
